LOCUS       pLV-hU6-CMV-green        7783 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5704..6564
                     /gene=Amp(R)
     promoter        6765..7305
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4309..4543
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5118..5573)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4615..4745
                     /gene=SV40 pA
     misc_feature    4746..4959
                     /gene=SV40 ori
     promoter        2289..2820
                     /gene=CMV
     CDS             2900..3619
                     /gene=GFP
     stem_loop       1824..1875
                     /gene=shRNA
     promoter        complement(1876..2139)
                     /gene=human U6 promoter
                     
BASE COUNT     2030 a      1892 c      1922 g      1897 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt ttcgtccttt ccacaagata tataaagcca agaaatcgaa 
     1921 atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc 
     1981 aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt 
     2041 tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga 
     2101 aggaatcatg ggaaataggc cctcttcctg cccgaccttg aattcggccg gatccaactt 
     2161 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 
     2221 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga 
     2281 taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 
     2341 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 
     2401 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 
     2461 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 
     2521 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 
     2581 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 
     2641 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 
     2701 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 
     2761 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt 
     2821 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctag 
     2881 agatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 
     2941 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 
     3001 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 
     3061 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg 
     3121 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 
     3181 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 
     3241 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 
     3301 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 
     3361 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 
     3421 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 
     3481 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga 
     3541 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 
     3601 ggacgagctg tacaagtaaa gcggccgcgt cgacaatcaa cctctggatt acaaaatttg 
     3661 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 
     3721 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 
     3781 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 
     3841 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 
     3901 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 
     3961 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 
     4021 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 
     4081 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 
     4141 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 
     4201 ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact 
     4261 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 
     4321 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac 
     4381 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 
     4441 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 
     4501 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 
     4561 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 
     4621 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 
     4681 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 
     4741 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 
     4801 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 
     4861 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt 
     4921 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 
     4981 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 
     5041 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 
     5101 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 
     5161 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 
     5221 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 
     5281 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 
     5341 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 
     5401 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 
     5461 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 
     5521 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc 
     5581 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 
     5641 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 
     5701 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 
     5761 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 
     5821 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 
     5881 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 
     5941 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 
     6001 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 
     6061 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 
     6121 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 
     6181 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 
     6241 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 
     6301 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 
     6361 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 
     6421 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 
     6481 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 
     6541 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 
     6601 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
     6661 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     6721 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     6781 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     6841 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     6901 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     6961 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     7021 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     7081 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     7141 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     7201 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     7261 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     7321 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     7381 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     7441 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     7501 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     7561 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 
     7621 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 
     7681 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag 
     7741 cgcgcaatta accctcacta aagggaacaa aagctggagc tgc 
//
