LOCUS       pLV-H1-mPGK-green        7662 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5583..6443
                     /gene=Amp(R)
     promoter        6644..7184
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4188..4422
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(4997..5452)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4494..4624
                     /gene=SV40 pA
     misc_feature    4625..4838
                     /gene=SV40 ori
     promoter        2241..2687
                     /gene=mouse PGK promoter
     CDS             2779..3498
                     /gene=GFP
     stem_loop       1824..1875
                     /gene=shRNA
     promoter        complement(1876..2104)
                     /gene=H1 promoter
                     
BASE COUNT     1925 a      1921 c      1959 g      1815 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt agagtggtct catacagaac ttataagatt cccaaatcca 
     1921 aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg 
     1981 gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt 
     2041 cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga 
     2101 attcggccgg atccaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 
     2161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 
     2221 aaattcaaaa ttttatcgat aattctaccg ggtaggggag gcgcttttcc caaggcagtc 
     2281 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc 
     2341 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt 
     2401 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc 
     2461 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac 
     2521 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt 
     2581 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct 
     2641 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa 
     2701 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgtctaga 
     2761 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 
     2821 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 
     2881 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 
     2941 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 
     3001 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 
     3061 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 
     3121 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 
     3181 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 
     3241 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 
     3301 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 
     3361 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 
     3421 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 
     3481 gacgagctgt acaagtaaag cggccgcgtc gacaatcaac ctctggatta caaaatttgt 
     3541 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 
     3601 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 
     3661 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 
     3721 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 
     3781 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 
     3841 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 
     3901 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 
     3961 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 
     4021 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 
     4081 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt 
     4141 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 
     4201 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 
     4261 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 
     4321 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 
     4381 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 
     4441 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 
     4501 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     4561 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     4621 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 
     4681 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 
     4741 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta 
     4801 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt 
     4861 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 
     4921 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 
     4981 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 
     5041 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 
     5101 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 
     5161 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 
     5221 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 
     5281 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 
     5341 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 
     5401 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca 
     5461 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 
     5521 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 
     5581 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 
     5641 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 
     5701 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 
     5761 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 
     5821 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 
     5881 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 
     5941 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 
     6001 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 
     6061 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 
     6121 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 
     6181 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 
     6241 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 
     6301 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 
     6361 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 
     6421 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 
     6481 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 
     6541 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 
     6601 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 
     6661 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 
     6721 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 
     6781 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
     6841 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
     6901 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
     6961 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
     7021 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
     7081 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
     7141 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
     7201 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
     7261 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 
     7321 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 
     7381 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 
     7441 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 
     7501 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 
     7561 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc 
     7621 gcgcaattaa ccctcactaa agggaacaaa agctggagct gc 
//
