LOCUS       pLV-H1-EF1a-green        8538 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             6459..7319
                     /gene=Amp(R)
     promoter        7520..8060
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             5064..5298
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5873..6328)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    5370..5500
                     /gene=SV40 pA
     misc_feature    5501..5714
                     /gene=SV40 ori
     promoter        2241..2636
                     /gene=human EF1a promoter
     intron          2669..3611
                     /gene=intron
     CDS             3655..4374
                     /gene=GFP
     stem_loop       1824..1875
                     /gene=shRNA
     promoter        complement(1876..2104)
                     /gene=H1 promoter
                     
BASE COUNT     2083 a      2145 c      2236 g      2032 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt agagtggtct catacagaac ttataagatt cccaaatcca 
     1921 aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg 
     1981 gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt 
     2041 cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga 
     2101 attcggccgg atccaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 
     2161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 
     2221 aaattcaaaa ttttatcgat gcctccccgt caccaccccc cccaacccgc cccgaccgga 
     2281 gctgagagta attcatacaa aaggactcgc ccctgccttg gggaatccca gggaccgtcg 
     2341 ttaaactccc actaacgtag aacccagaga tcgctgcgtt cccgccccct cacccgcccg 
     2401 ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc 
     2461 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 
     2521 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 
     2581 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 
     2641 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 
     2701 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt 
     2761 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 
     2821 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 
     2881 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 
     2941 tttaaaattt ttgatgatat cctgcgacgc tttttttctg gcaagatagt cttgtaaatg 
     3001 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 
     3061 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 
     3121 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 
     3181 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 
     3241 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 
     3301 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 
     3361 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 
     3421 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 
     3481 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 
     3541 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 
     3601 ttccatttca ggtgtcgtga aaactacccc tctagagatc caccggtcgc caccatggtg 
     3661 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 
     3721 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 
     3781 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 
     3841 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 
     3901 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 
     3961 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 
     4021 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 
     4081 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 
     4141 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 
     4201 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 
     4261 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 
     4321 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc 
     4381 cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 
     4441 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 
     4501 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 
     4561 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 
     4621 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 
     4681 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 
     4741 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 
     4801 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 
     4861 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 
     4921 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 
     4981 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 
     5041 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc 
     5101 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 
     5161 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 
     5221 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 
     5281 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 
     5341 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 
     5401 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 
     5461 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 
     5521 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     5581 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 
     5641 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg 
     5701 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 
     5761 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 
     5821 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc 
     5881 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 
     5941 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 
     6001 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 
     6061 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 
     6121 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 
     6181 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 
     6241 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 
     6301 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa 
     6361 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 
     6421 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 
     6481 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 
     6541 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 
     6601 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 
     6661 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 
     6721 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 
     6781 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 
     6841 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 
     6901 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 
     6961 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 
     7021 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 
     7081 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 
     7141 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 
     7201 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 
     7261 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 
     7321 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 
     7381 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 
     7441 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 
     7501 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 
     7561 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 
     7621 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 
     7681 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 
     7741 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 
     7801 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 
     7861 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 
     7921 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 
     7981 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 
     8041 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 
     8101 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 
     8161 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 
     8221 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 
     8281 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 
     8341 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 
     8401 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 
     8461 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg 
     8521 aacaaaagct ggagctgc 
//
