LOCUS       pLV-H1-CMV-green        7742 bp    DNA     circular     23-JUN-2008

FEATURES             Location/Qualifiers
     CDS             5663..6523
                     /gene=Amp(R)
     promoter        6724..7264
                     /gene=pUC ori
     promoter        7..235
                     /gene=RSV
     LTR             4268..4502
                     /gene=3'LTR
     intron          526..1690
                     /gene=Intron
     rep_origin      complement(5077..5532)
                     /gene=f1 ori
     LTR             236..416
                     /gene=5'LTR
     polyA_signal    4574..4704
                     /gene=SV40 pA
     misc_feature    4705..4918
                     /gene=SV40 ori
     promoter        2248..2779
                     /gene=CMV
     CDS             2859..3578
                     /gene=GFP
     promoter        complement(1876..2104)
                     /gene=H1 promoter
     stem_loop       1824..1875
                     /gene=shRNA
                     
BASE COUNT     1992 a      1905 c      1942 g      1861 t        42 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 
     1861 nnnnnnnnnn nnnnnttttt agagtggtct catacagaac ttataagatt cccaaatcca 
     1921 aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg 
     1981 gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt 
     2041 cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga 
     2101 attcggccgg atccaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 
     2161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 
     2221 aaattcaaaa ttttatcgat aagcttggga gttccgcgtt acataactta cggtaaatgg 
     2281 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 
     2341 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 
     2401 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 
     2461 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 
     2521 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta 
     2581 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga 
     2641 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa 
     2701 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag 
     2761 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca 
     2821 tagaagacac cgactctaga gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc 
     2881 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 
     2941 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 
     3001 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 
     3061 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 
     3121 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 
     3181 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 
     3241 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 
     3301 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 
     3361 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 
     3421 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 
     3481 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 
     3541 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgtc gacaatcaac 
     3601 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 
     3661 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 
     3721 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 
     3781 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 
     3841 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 
     3901 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 
     3961 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 
     4021 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 
     4081 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 
     4141 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt 
     4201 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 
     4261 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac 
     4321 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 
     4381 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 
     4441 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 
     4501 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 
     4561 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     4621 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     4681 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg 
     4741 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     4801 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 
     4861 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac 
     4921 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 
     4981 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 
     5041 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 
     5101 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     5161 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     5221 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     5281 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     5341 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     5401 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     5461 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 
     5521 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     5581 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     5641 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     5701 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     5761 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     5821 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     5881 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     5941 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     6001 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     6061 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     6121 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     6181 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     6241 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     6301 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     6361 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     6421 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     6481 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     6541 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     6601 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     6661 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     6721 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     6781 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     6841 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     6901 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     6961 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     7021 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     7081 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     7141 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     7201 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     7261 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     7321 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
     7381 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 
     7441 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
     7501 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
     7561 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 
     7621 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 
     7681 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct 
     7741 gc 
//
