LOCUS       pLV-miR-Locker          9088 bp    DNA     circular      24-AUG-2010
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7009..7869
                     /label=Amp(R)
     promoter        8070..8610
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5614..5848
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6423..6878)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5920..6050
                     /label=SV40 pA
     misc_feature    6051..6264
                     /label=SV40 ori
     misc_feature    4490..5086
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             3347..4480
                     /label=GFP-Bsd
     promoter        5109..5423
                     /label=mU6 promoter
     misc_feature    5449..5498
                     /label=mir-locker
BASE COUNT     2215 a      2278 c      2373 g      2178 t        44 others
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagaga tccaccggtc gccaccatgg tgagcaaggg 
     3361 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 
     3421 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 
     3481 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 
     3541 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 
     3601 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 
     3661 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 
     3721 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 
     3781 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 
     3841 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 
     3901 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 
     3961 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 
     4021 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggacccac cggtcgccac 
     4081 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat 
     4141 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg 
     4201 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt 
     4261 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg 
     4321 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct 
     4381 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat 
     4441 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcggccgcgt cgacaatcaa 
     4501 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 
     4561 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 
     4621 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 
     4681 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 
     4741 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 
     4801 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 
     4861 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 
     4921 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 
     4981 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 
     5041 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctcgagtct 
     5101 gaggatccga tccgacgccg ccatctctag gcccgcgccg gccccctcgc acagacttgt 
     5161 gggagaagct cggctactcc cctgccccgg ttaatttgca tataatattt cctagtaact 
     5221 atagaggctt aatgtgcgat aaaagacaga taatctgttc tttttaatac tagctacatt 
     5281 ttacatgata ggcttggatt tctataagag atacaaatac taaattatta ttttaaaaaa 
     5341 cagcacaaaa ggaaactcac cctaactgta aagtaattgt gtgttttgag actataaata 
     5401 tcccttggag aaaagccttg tttgtgctcg cttcggcgag cactctagnn nnnnnnnnnn 
     5461 nnnnnnnnnn ggatccnnnn nnnnnnnnnn nnnnnnnnag cttggagcgg acttcggtcc 
     5521 gctccttttt tttactagtc aacggtacct ttaagaccaa tgacttacaa ggcagctgta 
     5581 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 
     5641 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 
     5701 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 
     5761 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 
     5821 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 
     5881 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 
     5941 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 
     6001 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 
     6061 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     6121 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     6181 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta 
     6241 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa 
     6301 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 
     6361 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 
     6421 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     6481 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     6541 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 
     6601 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 
     6661 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     6721 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 
     6781 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     6841 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 
     6901 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 
     6961 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 
     7021 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 
     7081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 
     7141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 
     7201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 
     7261 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 
     7321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 
     7381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 
     7441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 
     7501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 
     7561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 
     7621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 
     7681 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 
     7741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 
     7801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 
     7861 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 
     7921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 
     7981 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 
     8041 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 
     8101 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 
     8161 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 
     8221 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 
     8281 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 
     8341 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 
     8401 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 
     8461 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 
     8521 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 
     8581 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 
     8641 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 
     8701 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 
     8761 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 
     8821 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 
     8881 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 
     8941 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 
     9001 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct 
     9061 cactaaaggg aacaaaagct ggagctgc 
//
