LOCUS       pLV-EF1a-MCS-IRES-RFP-Neo    9695 bp    DNA     circular     15-AUG-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7616..8476
                     /label=Amp(R)
     promoter        8677..9217
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6221..6455
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7030..7485)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    6658..6871
                     /label=SV40 ori
     misc_feature    5542..6138
                     /label=WPRE
     promoter        1933..2327
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             4012..5532
                     /label=RFP-Neo
     RBS             3401..3985
                     /label=IRES
BASE COUNT     2307 a      2471 c      2618 g      2299 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 
     3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 
     3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 
     3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 
     3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 
     3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 
     3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 
     3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 
     3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 
     3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 
     3961 aaaacacgat gataatatgg ccacactaga gtcgagctac cggtcgccac catggtgagc 
     4021 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg 
     4081 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 
     4141 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg 
     4201 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac 
     4261 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 
     4321 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc 
     4381 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag 
     4441 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag 
     4501 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 
     4561 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 
     4621 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 
     4681 ggccgccact ccaccggcgg catggacgag ctgtacaagg acccaccggt cgccaccatg 
     4741 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 
     4801 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 
     4861 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 
     4921 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 
     4981 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 
     5041 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 
     5101 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 
     5161 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 
     5221 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 
     5281 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 
     5341 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 
     5401 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 
     5461 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 
     5521 gagttcttct gagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat 
     5581 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 
     5641 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 
     5701 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 
     5761 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 
     5821 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 
     5881 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 
     5941 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 
     6001 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 
     6061 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 
     6121 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc 
     6181 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 
     6241 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 
     6301 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 
     6361 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 
     6421 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 
     6481 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 
     6541 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     6601 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 
     6661 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 
     6721 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 
     6781 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt 
     6841 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact 
     6901 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 
     6961 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 
     7021 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 
     7081 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 
     7141 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 
     7201 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 
     7261 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 
     7321 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 
     7381 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 
     7441 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg 
     7501 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 
     7561 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 
     7621 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 
     7681 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 
     7741 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 
     7801 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 
     7861 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 
     7921 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 
     7981 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 
     8041 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 
     8101 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 
     8161 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 
     8221 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 
     8281 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 
     8341 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 
     8401 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 
     8461 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 
     8521 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 
     8581 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 
     8641 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 
     8701 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 
     8761 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 
     8821 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 
     8881 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 
     8941 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 
     9001 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 
     9061 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 
     9121 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 
     9181 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 
     9241 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 
     9301 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 
     9361 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 
     9421 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 
     9481 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 
     9541 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 
     9601 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat 
     9661 taaccctcac taaagggaac aaaagctgga gctgc 
//
