LOCUS       pLV-EF1a-MCS-IRES-RFP-Bsd  9299 bp    DNA     circular     15-AUG-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7220..8080
                     /label=Amp(R)
     promoter        8281..8821
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5825..6059
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6634..7089)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    6262..6475
                     /label=SV40 ori
     misc_feature    5146..5742
                     /label=WPRE
     promoter        1933..2327
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     RBS             3401..3985
                     /label=IRES
     CDS             4012..5136
                     /label=RFP-Bsd
BASE COUNT     2252 a      2351 c      2483 g      2213 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 
     3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 
     3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 
     3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 
     3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 
     3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 
     3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 
     3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 
     3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 
     3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 
     3961 aaaacacgat gataatatgg ccacactaga gtcgagctac cggtcgccac catggtgagc 
     4021 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg 
     4081 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 
     4141 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg 
     4201 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac 
     4261 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 
     4321 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc 
     4381 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag 
     4441 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag 
     4501 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 
     4561 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 
     4621 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 
     4681 ggccgccact ccaccggcgg catggacgag ctgtacaagg acccaccggt cgccaccatg 
     4741 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac 
     4801 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc 
     4861 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg 
     4921 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat 
     4981 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat 
     5041 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt 
     5101 gaattgctgc cctctggtta tgtgtgggag ggctaagcgg ccgcgtcgac aatcaacctc 
     5161 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 
     5221 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 
     5281 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 
     5341 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 
     5401 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 
     5461 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 
     5521 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 
     5581 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 
     5641 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 
     5701 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc 
     5761 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 
     5821 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 
     5881 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 
     5941 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 
     6001 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 
     6061 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 
     6121 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     6181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     6241 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc 
     6301 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 
     6361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 
     6421 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg 
     6481 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 
     6541 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 
     6601 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg 
     6661 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
     6721 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 
     6781 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 
     6841 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 
     6901 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 
     6961 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 
     7021 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 
     7081 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 
     7141 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 
     7201 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 
     7261 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 
     7321 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 
     7381 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 
     7441 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 
     7501 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 
     7561 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 
     7621 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 
     7681 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 
     7741 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 
     7801 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 
     7861 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 
     7921 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 
     7981 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 
     8041 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 
     8101 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 
     8161 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 
     8221 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 
     8281 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 
     8341 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 
     8401 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 
     8461 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 
     8521 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 
     8581 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 
     8641 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 
     8701 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 
     8761 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 
     8821 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 
     8881 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 
     8941 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 
     9001 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 
     9061 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 
     9121 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 
     9181 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 
     9241 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc 
//
