LOCUS       pLV-EF1a-MCS-IRES-GFP-Bsd    9305 bp    DNA     circular     15-AUG-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7226..8086
                     /label=Amp(R)
     promoter        8287..8827
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5831..6065
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      6640..7095
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    6268..6481
                     /label=SV40 ori
     misc_feature    5152..5748
                     /label=WPRE
     promoter        1933..2327
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     RBS             3401..3985
                     /label=IRES
     CDS             4009..5142
                     /label=GFP-Bsd
BASE COUNT     2261 a      2366 c      2464 g      2214 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 
     3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 
     3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 
     3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 
     3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 
     3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 
     3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 
     3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 
     3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 
     3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 
     3961 aaaacacgat gataatatgg ccacactaga gatccaccgg tcgccaccat ggtgagcaag 
     4021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 
     4081 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 
     4141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 
     4201 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 
     4261 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 
     4321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 
     4381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 
     4441 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 
     4501 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 
     4561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 
     4621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 
     4681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggaccc accggtcgcc 
     4741 accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca 
     4801 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac 
     4861 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc 
     4921 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc 
     4981 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat 
     5041 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg 
     5101 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagcggccgc gtcgacaatc 
     5161 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 
     5221 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 
     5281 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 
     5341 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 
     5401 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 
     5461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 
     5521 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 
     5581 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 
     5641 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 
     5701 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc 
     5761 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 
     5821 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg 
     5881 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 
     5941 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 
     6001 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 
     6061 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 
     6121 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     6181 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     6241 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc 
     6301 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 
     6361 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 
     6421 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct 
     6481 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 
     6541 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 
     6601 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat 
     6661 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 
     6721 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 
     6781 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 
     6841 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 
     6901 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 
     6961 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 
     7021 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 
     7081 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
     7141 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     7201 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 
     7261 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 
     7321 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 
     7381 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 
     7441 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 
     7501 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 
     7561 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 
     7621 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 
     7681 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 
     7741 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 
     7801 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 
     7861 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 
     7921 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 
     7981 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 
     8041 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 
     8101 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     8161 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     8221 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     8281 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     8341 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     8401 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     8461 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     8521 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     8581 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     8641 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     8701 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     8761 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     8821 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     8881 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     8941 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 
     9001 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 
     9061 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 
     9121 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 
     9181 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 
     9241 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga 
     9301 gctgc 
//
