LOCUS       pLV-EF1a-MCS-IRES-GFP   8892 bp    DNA     circular     22-MAY-2007
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             6813..7673
                     /label=Amp(R)
     promoter        7874..8414
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5418..5652
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      6227..6682
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    5855..6068
                     /label=SV40 ori
     misc_feature    4739..5335
                     /label=WPRE
     promoter        1933..2327
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             4009..4728
                     /label=EGFP
     RBS             3401..3985
                     /label=IRES
BASE COUNT     2172 a      2251 c      2346 g      2123 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 
     3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 
     3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 
     3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 
     3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 
     3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 
     3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 
     3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 
     3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 
     3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 
     3961 aaaacacgat gataatatgg ccacactaga gatccaccgg tcgccaccat ggtgagcaag 
     4021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 
     4081 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 
     4141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 
     4201 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 
     4261 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 
     4321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 
     4381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 
     4441 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 
     4501 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 
     4561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 
     4621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 
     4681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgtc 
     4741 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 
     4801 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 
     4861 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 
     4921 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 
     4981 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 
     5041 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 
     5101 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg 
     5161 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 
     5221 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 
     5281 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt 
     5341 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 
     5401 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt 
     5461 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 
     5521 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 
     5581 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 
     5641 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 
     5701 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 
     5761 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     5821 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 
     5881 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 
     5941 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 
     6001 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac 
     6061 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 
     6121 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 
     6181 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc 
     6241 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 
     6301 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 
     6361 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 
     6421 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 
     6481 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 
     6541 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 
     6601 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 
     6661 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta 
     6721 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 
     6781 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 
     6841 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 
     6901 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 
     6961 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 
     7021 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 
     7081 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 
     7141 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 
     7201 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 
     7261 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 
     7321 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 
     7381 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 
     7441 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 
     7501 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 
     7561 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 
     7621 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 
     7681 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 
     7741 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 
     7801 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 
     7861 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 
     7921 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 
     7981 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 
     8041 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 
     8101 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 
     8161 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 
     8221 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 
     8281 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 
     8341 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 
     8401 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 
     8461 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 
     8521 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 
     8581 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 
     8641 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 
     8701 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 
     8761 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 
     8821 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa 
     8881 agctggagct gc 
//
