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Stem Cell Research

Lenti iPSC Plasmids for Stem Cell Research

Reprogramming mouse and human somatic cells to pluripotent stem cells by expressing a combination of transcription factors has been achieved in several laboratories since 2006 [1-4]. Biosettia has cloned six defined reprogramming factors, Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28, into lentiviral expression pLV-iPSC vectors.

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Figure 1. Biosettia’s pLV-iPSC vectors.

iPSC Lentiviral Vectors: each plasmid DNA in 1 ml of bacterial glycerol stock.

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Cat# Product Name Price Qty Add to cart
iPSC-p01 pLV-EF1a-hOct4-IRES-Bsd $ 575.00
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iPSC-p02 pLV-EF1a-hOct4-IRES-Hyg $ 575.00
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iPSC-p03 pLV-EF1a-hOct4-IRES-Neo $ 575.00
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iPSC-p04 pLV-EF1a-hSox2-IRES-Bsd $ 575.00
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iPSC-p05 pLV-EF1a-hSox2-IRES-Hyg $ 575.00
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iPSC-p06 pLV-EF1a-hSox2-IRES-Neo $ 575.00
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iPSC-p07 pLV-EF1a-hNanog-IRES-Bsd $ 575.00
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iPSC-p08 pLV-EF1a-hNanog-IRES-Puro $ 575.00
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iPSC-p09 pLV-EF1a-hLin28-IRES-Bsd $ 575.00
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iPSC-p10 pLV-EF1a-hLin28-IRES-Neo $ 575.00
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iPSC-p11 pLV-EF1a-hKlf4-IRES-Bsd $ 575.00
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iPSC-p12 pLV-EF1a-hKlf4-IRES-Neo $ 575.00
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iPSC-p13 pLV-EF1a-hKlf4-IRES-Puro $ 575.00
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iPSC-p14 pLV-EF1a-hc-Myc-IRES-Bsd $ 575.00
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iPSC Lentiviral Vector Set: each plasmid DNA in 1 ml of bacterial glycerol stock.

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Cat# Product Name Price Qty Add to cart
iPSC-pset01 Human pLV-iPSC Lentiviral Vector Set-1 (Oct4, Sox2, Nanog, Lin28) $ 1,750.00
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iPSC-pset02 Human pLV-iPSC Lentiviral Vector Set-2 (Oct4, Sox2, Klf4, c-Myc) $ 1,750.00
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iPSC-pset03 Human pLV-iPSC Lentiviral Vector Set-3 (Oct4, Sox2, Nanog, Lin28, Klf4, c-Myc) $ 2,375.00
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iPSC Polycistronic Lentiviral Vector: plasmid DNA in 1 ml of bacterial glycerol stock.

All four human reprogramming genes are linked using different self-cleaving 2A peptides and co-expressed the selectable markers via either F2A or internal ribosomal entry sequences (IRES) from the EF1a promoter.
Vector abbreviations:
SOKM: Sox2-P2A-Oct4-T2A-Klf4-E2A-c-Myc
SOKMGB: SOKM-F2A-GFP-Bsd
SOKMIB: SOKM-IRES-Bsd
SOKMIGB: SOKM-IRES-GFP-Bsd
SOKMIRB: SOKM-IRES-RFP-Bsd
SOKMIRP: SOKM-IRES-RFP-Puro
SOKMIRN: SOKM-IRES-RFP-Neo
SONL: Sox2-P2A-Oct4-T2A-Nanog-E2A-Lin28
SONLGB: SONL-F2A-GFP-Bsd
SONLIB: SONL-IRES-Bsd
SONLIGB: SONL-IRES-GFP-Bsd
SONLIRB: SONL-IRES-RFP-Bsd
SONLIRP: SONL-IRES-RFP-Puro
SONLIRN: SONL-IRES-RFP-Neo

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Cat# Product Name Price Qty Add to cart
iPSC-p4F01 pLV-iPSC-SOKMGB $ 1,680.00
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iPSC-p4F02 pLV-iPSC-SOKMIB $ 1,490.00
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iPSC-p4F03 pLV-iPSC-SOKMIGB $ 1,590.00
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iPSC-p4F04 pLV-iPSC-SOKMIRB $ 1,590.00
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iPSC-p4F05 pLV-iPSC-SOKMIRP $ 1,590.00
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iPSC-p4F06 pLV-iPSC-SOKMIRN $ 1,590.00
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iPSC-p4F07 pLV-iPSC-SONLGB $ 1,680.00
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iPSC-p4F08 pLV-iPSC-SONLIB $ 1,490.00
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iPSC-p4F09 pLV-iPSC-SONLIGB $ 1,590.00
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iPSC-p4F10 pLV-iPSC-SONLIRB $ 1,590.00
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iPSC-p4F11 pLV-iPSC-SONLIRP $ 1,590.00
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iPSC-p4F12 pLV-iPSC-SONLIRN $ 1,590.00
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iPSC-p4F13 pLV-iPSC-SOKGB $ 1,550.00
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Cat# Product Name Price Qty Add to cart
iPSC-v4F01m LV-iPSC-SOKMGB $ 1,890.00
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iPSC-v4F02m LV-iPSC-SOKMIB $ 1,890.00
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iPSC-v4F03m LV-iPSC-SOKMIGB $ 1,890.00
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iPSC-v4F04m LV-iPSC-SOKMIRB $ 1,890.00
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iPSC-v4F05m LV-iPSC-SOKMIRP $ 1,890.00
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iPSC-v4F06m LV-iPSC-SOKMIRN $ 1,890.00
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iPSC-v4F07m LV-iPSC-SONLGB $ 1,890.00
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iPSC-v4F08m LV-iPSC-SONLIB $ 1,890.00
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iPSC-v4F09m LV-iPSC-SONLIGB $ 1,890.00
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iPSC-v4F10m LV-iPSC-SONLIRB $ 1,890.00
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iPSC-v4F11m LV-iPSC-SONLIRP $ 1,890.00
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iPSC-v4F12m LV-iPSC-SONLIRN $ 1,890.00
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LEFT: Morphology of ES-like iPS(MEF) colonies generated by pLV-iPSC-SOKMGB transduction. RIGHT: Morphology of ES-like iPS(IMR90) colonies stained with alkaline phosphatase.

Biosettia has provided additional iPSC vector/lentivirus support to our clients through a variety of custom services. To inquire about inquire about these services or to request a quote, please e-mail us at info@biosettia.com