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FavorPrep Plant Total RNA Maxi Kit (10)

FavorPrep Plant Total RNA Maxi Kit (10)

 FavorPrep™ Plant Total RNA Maxi Kit provides a fast and simple method to isolate total RNA from plant tissue and cells. In the process, the sample is homogenized by grinding plant tissue in liquid nitrogen and filtrated by filter column to remove cell debris. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to glass fiber matrix in the spin column. Optional DNase treatments can remove DNA residues and the contaminants are washed with an ethanol contained wash buffer. Finally, the purified total RNA is eluted by RNase-free water. The protocol does not require phenol extraction and alcohol precipitation. The entire procedure can be completed in 60 minutes. The purified total RNA is ready for RT, RT-PCR, real-time PCR, and Northern blotting.


Sample: 500 mg (up to 1 g) plant tissue or 5~10 X 10^7 plant cells
Yield: up to 50~300 µg total RNA from young leave
Operation Time: about 45~60 min
Binding Capacity: up to 1000 µg total RNA
Elution volume: 500 µl
Download Protocol


Components:
FARB Buffer, FAPRB Buffer, Wash Buffer 1, Wash Buffer 2 (Concentrated), RNase-free Water, Filter Column, FARB Maxi Column

Cat#: FAPRK002
$ 230.00Price:
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