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pLV-[hsa-mir-17_18a _19a_20a_19b-1 _92a-1] plasmid

pLV-[hsa-mir-17_18a _19a_20a_19b-1 _92a-1] plasmid

miRNA Plasmid MapHuman microRNA (hsa-mir) precursors and approximately 100-bp upstream and downstream flanking genomic sequences were PCR amplified and cloned into a self-inactivated (SIN) lentiviral vector to generate pLV-miRNA vectors. The cloning site of pre-miRNA genomic fragments is within the intron of human EF1a promoter region. The miRNA of interest can be delivered into cells by transient transfection of the pLV-miRNA plasmid or lentiviral transduction while the miRNA lentiviral stock is prepared from cotransfecting HEK 293T cells with the pLV-miRNA plasmid and lentiviral packaging vector mix.

Cat#: mir-pc02
$ 675.00Price:
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