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Product Details and Specifications
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Product Name |
Firefly Luciferase (FLuc)-IRES-Puro Lentivirus |
Product Catalog Number |
GlowCell-14p-1 (1ml), GlowCell-14p-5 (5ml), GlowCell-14p-10 (10ml) |
Price |
$450 (1ml), $1,575 (5ml), $2,250 (10ml) |
Components |
1, 5, or 10 mL Firefly Luciferase Lentivirus |
Titer |
≥1x107 IU/mL (in HT1080 cells) |
Target Location |
Whole Cell |
Storage Conditions |
Store at -70C |
Shipping Conditions |
Our lentiviruses are shipped on dry ice via overnight delivery. Please note that additional shipping charges may apply. |
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About Luciferase Lentivirus
Luciferase enzymes are used in bioluminescence and have become powerful tools in biological and medical research. These enzymes have been discovered in several animal species, and due to their variance in spectral properties, luciferase types can be used singly or in combination with each other. For example, Gaussia and Renilla type luciferase enzymes are classified as emitting “flash” light (Figure 1) due to its high-intensity and rapid-decay light. On the other hand, Firefly luciferase enzymes exhibit “glow” kinetics, characterized by less sensitive but longer lasting emittance. A table summarizing some of these difference is shown below (Table 1). Therefore, it is important to know which types of luciferase is needed for your experiments. We provide lentiviruses with an optional selection marker for the expression of Renilla and Firefly-type luciferase, which can be used in single reporter experiments. Additionally, we have made available Renilla-T2A-Firefly luciferase lentiviruses for duel reporter assays.
Comparing Luciferase
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Firefly Luciferase |
Renilla Luciferase |
Gaussia Luciferase |
Substrate |
Luciferin |
Coelenterazine |
Coelenterazine |
Cofactor |
C02,ATP,Mg2+ |
O2 |
O2 |
Light |
Green-Yellow |
Blue |
Blue |
Wave Length |
550-570nm |
470-490nm |
470-490nm |
Secretion |
No |
No |
Yes |
Kinetics |
Glow |
Flash |
Flash |
Glowcell-14n-10 Firefly Luciferase (FLuc)-IRES-Neo Lentivirus |
Glowcell-14b-10 Firefly Luciferase (FLuc)-IRES-Bsd Lentivirus |
Glowcell-14h-10 Firefly Luciferase (FLuc)-IRES-Hyg Lentivirus |
Glowcell-14p-10 Firefly Luciferase (FLuc)-IRES-Puro Lentivirus |
Glowcell-15-10 Firefly Luciferase (FLuc)-T2A-RFP Lentivirus |
Glowcell-15b-10 Firefly Luciferase (FLuc)-T2A-RFP-IRES-Bsd Lentivirus |
Glowcell-15h-10 Firefly Luciferase (FLuc)-T2A-RFP-IRES-Hyg Lentivirus |
Glowcell-15p-10 Firefly Luciferase (FLuc)-T2A-RFP-IRES-Puro Lentivirus |
Glowcell-16-10 Firefly Luciferase (FLuc)-F2A-GFP Lentivirus |
Glowcell-16b-10 Firefly Luciferase (FLuc)-F2A-GFP-IRES-Bsd Lentivirus |
Glowcell-16h-10 Firefly Luciferase (FLuc)-F2A-GFP-IRES-Hyg Lentivirus |
Glowcell-16p-10 Firefly Luciferase (FLuc)-F2A-GFP-IRES-Puro Lentivirus |
Glowcell-20n-10 Renilla Luciferase (RLuc)-IRES-Neo Lentivirus |
Glowcell-20b-10 Renilla Luciferase (RLuc)-IRES-Bsd Lentivirus |
Glowcell-20h-10 Renilla Luciferase (RLuc)-IRES-Hyg Lentivirus |
Glowcell-20p-10 Renilla Luciferase (RLuc)-IRES-Puro Lentivirus |
Glowcell-21-10 Renilla Luciferase (RLuc)-T2A-RFP Lentivirus |
Glowcell-21b-10 Renilla Luciferase (RLuc)-T2A-RFP-IRES-Bsd Lentivirus |
Glowcell-21h-10 Renilla Luciferase (RLuc)-T2A-RFP-IRES-Hyg Lentivirus |
Glowcell-21p-10 Renilla Luciferase (RLuc)-T2A-RFP-IRES-Puro Lentivirus |
Glowcell-22-10 Renilla Luciferase (RLuc)-F2A-GFP Lentivirus |
Glowcell-22b-10 Renilla Luciferase (RLuc)-F2A-GFP-IRES-Bsd Lentivirus |
Glowcell-22h-10 Renilla Luciferase (RLuc)-F2A-GFP-IRES-Hyg Lentivirus |
Glowcell-22p-10 Renilla Luciferase (RLuc)-F2A-GFP-IRES-Puro Lentivirus |
Glowcell-24-10 Renilla-Firefly (RLuc)-T2A-(FLuc)-F2A-GFP-Bsd Lentivirus |
Firefly/Renilla Luciferase with drug selection: |
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Firefly/Renilla Luciferase with RFP and optional drug selection: |
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Firefly/Renilla Luciferase with GFP and optional drug selection: |
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