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pLV-EF1a-hKlf4-IRES-Puro

pLV-EF1a-hKlf4-IRES-Puro

Product Details and Specifications
Product Name pLV-EF1a-hKlf4-IRES-Puro
Catalog Number iPSC-p13
Price 575.00 US Dollars
Components Each kit comes with DNA provided in 1ml of bacterial glycerol stock
Storage Conditions Store your iPSC vector at -70 to -80 degrees Celsius
Shipping Conditions Our iPSC vectors at room temperature via overnight delivery. Please note that additional shipping charges may apply.
Map Download Genbank
Protocol Download PDF

Product Description

Reprogramming mouse and human somatic cells to pluripotent stem cells by expressing a combination of transcription factors have been achieved in several laboratories since 2006. Biosettia has cloned six defined reprogramming factors, Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28, each into lentiviral expression pLV-iPSC vectors.

Safety Guidelines for Working with Lenti-iPSC

The recombinant lentiviruses have been designated as Level 2 organisms by NIH and CDC. ABiosafety Level 2 (BSL-2) facility is required in order to work with lentiviruses. The information of Biosafety in Microbiological and Biomedical Laboratories(BMBL) can be downloaded from the following link: www.cdc.gov/od/ohs/biosfty/bmbl5/BMBL_5th_Edition.pdf

Catalog Number Name Reprogramming Factor Download Sequence
iPSC-p01 pLV-EF1a-hOct4-IRES-Bsd Oct4 Genbank
iPSC-p02 pLV-EF1a-hOct4-IRES-Hyg Oct4 Genbank
iPSC-P03 pLV-EF1a-hOct4-IRES-Neo Oct4 Genbank
iPSC-P04 pLV-EF1a-hSox2-IRES-Bsd Sox2 Genbank
iPSC-P05 pLV-EF1a-hSox2-IRES-Hyg Sox2 Genbank
iPSC-P06 pLV-EF1a-hSox2-IRES-Neo Sox2 Genbank
iPSC-P07 pLV-EF1a-hNanog-IRES-Bsd Nanog Genbank
iPSC-P08 pLV-EF1a-hNanog-IRES-Puro Nanog Genbank
iPSC-P09 pLV-EF1a-hLin28-IRES-Bsd Lin28 Genbank
iPSC-P10 pLV-EF1a-hLin28-IRES-Neo Lin28 Genbank
iPSC-P11 pLV-EF1a-hKlf4-IRES-Bsd Klf4 Genbank
iPSC-P12 pLV-EF1a-hKlf4-IRES-Neo Klf4 Genbank
iPSC-P13 pLV-EF1a-hKlf4-IRES-Puro Klf4 Genbank
iPSC-P14 pLV-EF1a-hc-Myc-IRES-Bsd Myc Genbank



iPSC Lentiviral Vector Set

Researchers who may need more than one iPSC vector may be interested in Biosettia's iPSC Vector Sets, which include four to six vectors each containing a different reprogramming factor.



iPSC Polycistronic Lentiviral Vector

Biosettia provides vectors containing multiple reprogramming factors linked using different self-cleaving 2A peptides. Each polycistronic vector contains either one set of: (Sox2, Oct4, Kl4, and c-Myc) or (Sox2, Oct4, Nanog, and Lin28).



iPSC All-in-One Lentivirus

iPSC All-in-One Lentiviruses are provided as an alternative to Biosettia's polycistronic iPSC vectors. Each kit contains 10 ml of lentivirus with titer at least 1x106 IU/ml.

Cat#: iPSC-p13
$ 575.00Price:
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