pLV-EF1a-MCS-IRES-RFP-Puro
Product Details and Specifications |
Product Name |
pLV-EF1a-MCS-IRES-RFP-Puro |
|
Product Catalog Number |
cDNA-pLV09 |
Components |
Each kit contains 40 ul plasmid DNA (250 ng/ul), 20 ul EF1a forward primer (10 uM), and 20 ul IRES reverse primer (10 uM) |
Selectable Marker |
RFP-Puro |
Storage Conditions |
Store your lentiviral gene expression vector and its sequencing primers at -20 degrees Celsius |
Shipping Conditions |
Our cDNA vectors are shipped at room temperature via overnight delivery. Please note that additional shipping charges may apply. |
Download Map |
Download Genbank |
Download Protocol |
Download PDF |
Product Description
|
The pLV-cDNA vector is a self-inactivated (SIN) vector carrying a deletion in the U3 region of the 3’ LTR, which eliminates the promoter activity of LTR. The self-inactivating deletion provides an additional level of safety by preventing the integrated viral genome from generating full-length lentiviral RNA. The pLV-cDNA vector can be used in transiently expressing gene of interest by transfection and long-term expression while the cells are transduced by packaged lentivirus. |
Safety Guidelines for Working with Lenti-cDNA
|
The recombinant lentiviruses have been designated as Level 2 organisms by NIH and CDC. ABiosafety Level 2 (BSL-2) facility is required in order to work with lentiviruses. The information of Biosafety in Microbiological and Biomedical Laboratories(BMBL) can be downloaded from the following link: www.cdc.gov/od/ohs/biosfty/bmbl5/BMBL_5th_Edition.pdf |
Purchase our cDNA Vector + miniprep package and also receive:
- 1 kit FavorPrep Plasmid DNA Extraction Kit (100 preps - $40.00 off retail price)
Cat#:
cDNA-pLV09