pLV-EF1a-MCS-IRES-RFP-Bsd
pLV-EF1a-MCS-IRES-RFP-Bsd
Product Details and Specifications | ||
Product Name | pLV-EF1a-MCS-IRES-RFP-Bsd | |
Product Catalog Number | cDNA-pLV08 | |
Components | Each kit contains 40 ul plasmid DNA (250 ng/ul), 20 ul EF1a forward primer (10 uM), and 20 ul IRES reverse primer (10 uM) | |
Selectable Marker | RFP-Bsd | |
Storage Conditions | Store your lentiviral gene expression vector and its sequencing primers at -20 degrees Celsius | |
Shipping Conditions | Our cDNA vectors are shipped at room temperature via overnight delivery. Please note that additional shipping charges may apply. | |
Download Map | Download Genbank | |
Download Protocol | Download PDF |
Product Description |
The pLV-cDNA vector is a self-inactivated (SIN) vector carrying a deletion in the U3 region of the 3’ LTR, which eliminates the promoter activity of LTR. The self-inactivating deletion provides an additional level of safety by preventing the integrated viral genome from generating full-length lentiviral RNA. The pLV-cDNA vector can be used in transiently expressing gene of interest by transfection and long-term expression while the cells are transduced by packaged lentivirus. |
Safety Guidelines for Working with Lenti-cDNA |
The recombinant lentiviruses have been designated as Level 2 organisms by NIH and CDC. ABiosafety Level 2 (BSL-2) facility is required in order to work with lentiviruses. The information of Biosafety in Microbiological and Biomedical Laboratories(BMBL) can be downloaded from the following link: www.cdc.gov/od/ohs/biosfty/bmbl5/BMBL_5th_Edition.pdf |
Catalog Number | Name | Marker | Download Sequence |
cDNA-pLV01 | pLV-EF1a-MCS-IRES-Puro | Puro | Download |
cDNA-pLV02 | pLV-EF1a-MCS-IRES-Hyg | Hyg | Download |
cDNA-pLV03 | pLV-EF1a-MCS-IRES-Bsd | Bsd | Download |
cDNA-pLV04 | pLV-EF1a-MCS-IRES-Neo | Neo | Download |
cDNA-pLV05 | pLV-EF1a-MCS-IRES-GFP | GFP | Download |
cDNA-pLV06 | pLV-EF1a-MCS-IRES-RFP | RFP | Download |
cDNA-pLV07 | pLV-EF1a-MCS-IRES-GFP-Bsd | GFP-Bsd | Download |
cDNA-pLV08 | pLV-EF1a-MCS-IRES-RFP-Bsd | RFP-Bsd | Download |
cDNA-pLV09 | pLV-EF1a-MCS-IRES-RFP-Puro | RFP-Puro | Download |
cDNA-pLV10 | pLV-EF1a-MCS-IRES-RFP-Neo | RFP-Neo | Download |
cDNA-pLV11 | pLV-EF1a-MCS-IRES-GFP-Puro | GFP-Puro | Download |
FavorPrep Plasmid DNA Extraction Mini Kit (100)
Favorgen's Plasmid DNA Extraction Kit provides a rapid, phenol-free method for the extraction of high-purity plasmid DNA from 1-6 ml of bacterial cultures.
Full Length cDNA Templates
Biosettia’s full-length cDNA templates are a perfect source for amplification of genes of interest. cDNA templates generated from different tissues and cell lines also provide an opportunity to researchers for characterization of alternatively spliced mRNA variants. In addition, the 3′-UTRs can be cloned for study of translational repression by specific miRNAs.