LOCUS pRNAi-mU6-green 4259 bp DNA circular 23-JUN-2008 FEATURES Location/Qualifiers rep_origin 3368..3956 /gene=pUC ori CDS 2337..3197 /gene=Amp(R) rep_origin 401..857 /gene=f1 ori promoter 864..1228 /gene=SV40 early promoter polyA_signal 1998..2128 /gene=SV40 early polyA promoter 16..330 /gene=mouse U6 promoter CDS 1259..1978 /gene=GFP insertion_seq 331..382 /gene=shRNA BASE COUNT 1105 a 1078 c 1052 g 982 t 42 others ORIGIN 1 cccgggacag aattcgatcc gacgccgcca tctctaggcc cgcgccggcc ccctcgcaca 61 gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct 121 agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag 181 ctacatttta catgataggc ttggatttct ataagagata caaatactaa attattattt 241 taaaaaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact 301 ataaatatcc cttggagaaa agcctaaaaa nnnnnnnnnn nnnnnnnnnn nttggatcca 361 annnnnnnnn nnnnnnnnnn nntttttttt aagcttcacc ttgtaagcgt taatattttg 421 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 481 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 541 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 601 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 661 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 721 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 781 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 841 ctacagggcg cgtcaggctc gagttctgag gcggaaagaa ccagctgtgg aatgtgtgtc 901 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 961 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 1021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 1081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 1141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 1201 ttggaggcct aggcttttgc aaaaagctcc cggtaatacg actcactata gggctagcat 1261 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 1321 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 1381 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 1441 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 1501 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 1561 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 1621 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 1681 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 1741 catcaaggtg aacttcgaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 1801 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 1861 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 1921 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc 1981 ggccgcaatc tagaccaaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2041 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2101 aactcatcaa tgtatcttat catgtctgtg atcaggtacc aaagggcctc gtgatacgcc 2161 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 2221 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 2281 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 2341 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 2401 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 2461 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 2521 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 2581 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 2641 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 2701 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 2761 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 2821 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 2881 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 2941 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 3001 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 3061 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 3121 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 3181 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 3241 aacttcattt ttaattaaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 3301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 3361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 3421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 3481 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 3541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 3601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 3661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 3721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 3781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 3841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 3901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 3961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 4021 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 4081 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 4141 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 4201 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctc //