LOCUS pRNAi-H1-bsd 3846 bp DNA circular 23-JUN-2008 FEATURES Location/Qualifiers rep_origin 2955..3543 /gene=pUC ori CDS 1924..2784 /gene=Amp(R) rep_origin 309..765 /gene=f1 ori promoter 772..1136 /gene=SV40 early promoter polyA_signal 1585..1715 /gene=SV40 early polyA promoter 10..238 /gene=H1 promoter CDS 1167..1565 /gene=Bsd(R) insertion_seq 239..290 /gene=shRNA BASE COUNT 975 a 928 c 971 g 930 t 42 others ORIGIN 1 cccgggacag aattcgaacg ctgacgtcat caacccgctc caaggaatcg cgggcccagt 61 gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc tgtgagggac 121 aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa 181 cgtgaaatgt ctttggattt gggaatctta taagttctgt atgagaccac tctaaaaann 241 nnnnnnnnnn nnnnnnnnnt tggatccaan nnnnnnnnnn nnnnnnnnnn ttttttttaa 301 gcttcacctt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggctcga gttctgaggc 781 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 961 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 1021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 1081 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 1141 gtaatacgac tcactatagg gctagcatgg ccaagccttt gtctcaagaa gaatccaccc 1201 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg 1261 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta 1321 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca 1381 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac 1441 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg 1501 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg 1561 gctaagcggc cgcaatctag accaaacttg tttattgcag cttataatgg ttacaaataa 1621 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 1681 ttgtccaaac tcatcaatgt atcttatcat gtctgtgatc aggtaccaaa gggcctcgtg 1741 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 1801 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 1861 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 1921 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 1981 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 2041 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 2101 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 2161 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 2221 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 2281 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 2341 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 2401 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 2461 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 2521 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 2581 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 2641 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 2701 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 2761 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 2821 gatttaaaac ttcattttta attaaaaagg atctaggtga agatcctttt tgataatctc 2881 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 2941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 3001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 3061 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 3121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 3181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 3241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 3301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 3361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 3421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 3481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 3541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 3601 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 3661 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 3721 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 3781 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 3841 tagctc //