LOCUS pLV-H1TetO-RFP-Bsd 10074 bp DNA circular 19-MAR-1980 SOURCE COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7995..8855 /label=Amp(R) promoter 9056..9596 /label=pUC\ori promoter 7..235 /label=RSV LTR 6600..6834 /label=3'\LTR intron 526..1690 /label=Intron rep_origin complement(7409..7864) /label=f1\ori LTR 236..416 /label=5'LTR polyA_signal 6906..7036 /label=SV40\pA misc_feature 7037..7250 /label=SV40\ori promoter 2104..2499 /label=human\EF1a\promoter intron 2532..3474 /label=intron promoter complement(1872..1971) /label=H1TetO CDS 3531..4172 /label=TetR RBS 4176..4177 /label=IRES stem_loop complement(1824..1871) /label=shRNA RBS 4176..4760 /label=IRES CDS 4787..5911 /label=RFP-Bsd BASE COUNT 2493 a 2491 c 2637 g 2415 t 38 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 1861 nnnnnnnnnn ntttttctct atcactgata gggaacttat aagtctctat cactgatagg 1921 gatttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tggatccaac 1981 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 2041 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc 2101 gatgcctccc cgtcaccacc ccccccaacc cgccccgacc ggagctgaga gtaattcata 2161 caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg 2221 tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag 2281 gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca 2341 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc 2401 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2461 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2521 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg 2581 gcccttgcgt gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc 2641 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg 2701 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg 2761 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga 2821 tatcctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac 2881 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca 2941 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa 3001 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg 3061 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct 3121 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc 3181 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac 3241 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt 3301 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt 3361 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct 3421 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg 3481 tgaaaactac ccctctagat aatacgactc actatagggc ccgggccacc atgtccagat 3541 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag 3601 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc 3661 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc 3721 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta 3781 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac 3841 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag 3901 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt 3961 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg 4021 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc 4081 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg 4141 aaagtgggtc cgcgtacagc ggctcccggt agctagcccc tctccctccc ccccccctaa 4201 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 4261 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 4321 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 4381 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 4441 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 4501 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 4561 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 4621 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc 4681 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 4741 acgatgataa tatggccaca ctagagtcga gctaccggtc gccaccatgg tgagcaaggg 4801 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 4861 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 4921 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 4981 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 5041 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 5101 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta 5161 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 5221 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 5281 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 5341 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 5401 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 5461 ccactccacc ggcggcatgg acgagctgta caaggaccca ccggtcgcca ccatggccaa 5521 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat 5581 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt 5641 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg 5701 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa 5761 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg 5821 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt 5881 gctgccctct ggttatgtgt gggagggcta agcggccgcg tcgacaatca acctctggat 5941 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 6001 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 6061 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 6121 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 6181 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 6241 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 6301 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 6361 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 6421 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 6481 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa 6541 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 6601 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc 6661 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6721 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6781 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6841 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 6901 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 7021 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac 7081 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 7141 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 7201 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc 7261 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 7321 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 7381 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg 7441 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 7501 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 7561 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 7621 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 7681 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 7741 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 7801 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca 7861 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 7921 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 7981 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 8041 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 8101 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 8161 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 8221 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 8281 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 8341 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 8401 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 8461 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 8521 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 8581 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 8641 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 8701 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 8761 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 8821 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 8881 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 8941 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 9001 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 9061 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 9121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 9181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 9241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 9301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 9361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 9421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 9481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 9541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 9601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 9661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 9721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 9781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 9841 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 9901 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 9961 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 10021 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgc //