LOCUS pLV-H1TetO-GFP-Bsd 10080 bp DNA circular 31-MAR-2010 SOURCE COMMENT COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 8001..8861 /label=Amp(R) promoter 9062..9602 /label=pUC ori promoter 7..235 /label=RSV LTR 6606..6840 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(7415..7870) /label=f1 ori LTR 236..416 /label=5'LTR polyA_signal 6912..7042 /label=SV40 pA misc_feature 7043..7256 /label=SV40 ori promoter 2104..2499 /label=human EF1a promoter intron 2532..3474 /label=intron promoter complement(1872..1971) /label=H1TetO CDS 3531..4172 /label=TetR RBS 4176..4760 /label=IRES CDS 4784..5917 /label=GFP-Bsd stem_loop complement(1824..1871) /label=shRNA BASE COUNT 2502 a 2506 c 2618 g 2416 t 38 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 1861 nnnnnnnnnn ntttttctct atcactgata gggaacttat aagtctctat cactgatagg 1921 gatttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tggatccaac 1981 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 2041 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc 2101 gatgcctccc cgtcaccacc ccccccaacc cgccccgacc ggagctgaga gtaattcata 2161 caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg 2221 tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag 2281 gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca 2341 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc 2401 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2461 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2521 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg 2581 gcccttgcgt gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc 2641 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg 2701 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg 2761 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga 2821 tatcctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac 2881 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca 2941 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa 3001 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg 3061 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct 3121 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc 3181 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac 3241 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt 3301 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt 3361 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct 3421 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg 3481 tgaaaactac ccctctagat aatacgactc actatagggc ccgggccacc atgtccagat 3541 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag 3601 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc 3661 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc 3721 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta 3781 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac 3841 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag 3901 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt 3961 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg 4021 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc 4081 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg 4141 aaagtgggtc cgcgtacagc ggctcccggt agctagcccc tctccctccc ccccccctaa 4201 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 4261 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 4321 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 4381 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 4441 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 4501 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 4561 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 4621 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc 4681 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 4741 acgatgataa tatggccaca ctagagatcc accggtcgcc accatggtga gcaagggcga 4801 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 4861 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 4921 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 4981 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 5041 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 5101 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 5161 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 5221 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 5281 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 5341 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 5401 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 5461 cgccgccggg atcactctcg gcatggacga gctgtacaag gacccaccgg tcgccaccat 5521 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa 5581 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg 5641 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt 5701 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa 5761 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca 5821 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg 5881 tgaattgctg ccctctggtt atgtgtggga gggctaagcg gccgcgtcga caatcaacct 5941 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 6001 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 6061 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 6121 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 6181 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 6241 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 6301 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 6361 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 6421 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 6481 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac 6541 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 6601 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg 6661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 6721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 6781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 6841 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 6901 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 6961 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 7021 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc 7081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 7141 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 7201 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg 7261 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 7321 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 7381 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 7441 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 7501 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 7561 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 7621 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 7681 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 7741 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 7801 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 7861 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 7921 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 7981 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 8041 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 8101 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 8161 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 8221 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 8281 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 8341 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 8401 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 8461 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 8521 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 8581 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 8641 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 8701 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 8761 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 8821 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 8881 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 8941 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 9001 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 9061 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 9121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 9181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 9241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 9301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 9361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 9421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 9481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 9541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 9601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 9661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 9721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 9781 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 9841 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 9901 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 9961 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 10021 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc //