LOCUS pLV-hU6-EF1a-GFP-Puro 9189 bp DNA circular 26-SEP-2010 SOURCE COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7110..7970 /label=Amp(R) promoter 8171..8711 /label=pUC ori promoter 7..235 /label=RSV LTR 5715..5949 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6524..6979) /label=f1 ori LTR 236..416 /label=5'LTR polyA_signal 6021..6151 /label=SV40 pA misc_feature 6152..6365 /label=SV40 ori promoter 2278..2673 /label=human EF1a promoter intron 2706..3648 /label=intron promoter complement(1872..2135) /label=human U6 CDS 3692..5026 /label=GFP-Puro stem_loop complement(1824..1871) /label=shRNA BASE COUNT 2208 a 2376 c 2424 g 2143 t 38 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 1861 nnnnnnnnnn ntttttttcg tcctttccac aagatatata aagccaagaa atcgaaatac 1921 tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac 1981 tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca 2041 gtcaaattaa ttctaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga 2101 atcatgggaa ataggccctc ttcctgcccg accttgaatt cggccggatc caacttttaa 2161 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2221 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgatgcc 2281 tccccgtcac cacccccccc aacccgcccc gaccggagct gagagtaatt catacaaaag 2341 gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact aacgtagaac 2401 ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac tgaggtggag 2461 aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc 2521 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg 2581 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 2641 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa 2701 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt 2761 gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga tcccgagctt 2821 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 2881 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 2941 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgatatcct 3001 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 3061 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 3121 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 3181 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 3241 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 3301 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 3361 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 3421 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 3481 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 3541 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 3601 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa 3661 ctacccctct agagatccac cggtcgccac catggtgagc aagggcgagg agctgttcac 3721 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 3781 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 3841 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 3901 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 3961 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 4021 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 4081 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 4141 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 4201 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 4261 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 4321 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 4381 cactctcggc atggacgagc tgtacaagga cccaccggtc gccaccatga ccgagtacaa 4441 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc 4501 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg 4561 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 4621 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 4681 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 4741 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 4801 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 4861 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 4921 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 4981 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagcgg ccgcgtcgac 5041 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 5101 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 5161 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 5221 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 5281 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 5341 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 5401 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 5461 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 5521 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 5581 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga 5641 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 5701 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg 5761 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 5821 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 5881 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 5941 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 6001 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 6061 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 6121 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 6181 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 6241 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 6301 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg 6361 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 6421 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 6481 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc 6541 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 6601 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 6661 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 6721 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 6781 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 6841 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 6901 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 6961 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 7021 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 7081 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 7141 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 7201 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 7261 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 7321 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 7381 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 7441 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 7501 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 7561 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 7621 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 7681 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 7741 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 7801 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 7861 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 7921 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 7981 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 8041 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 8101 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 8161 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 8221 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 8281 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 8341 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 8401 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 8461 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 8521 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 8581 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 8641 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 8701 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 8761 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 8821 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 8881 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 8941 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 9001 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 9061 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 9121 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc 9181 tggagctgc //