LOCUS pLV-H1-EF1a-GFP-Puro 9148 bp DNA circular 22-SEP-2010 SOURCE COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7069..7929 /label=Amp(R) promoter 8130..8670 /label=pUC ori promoter 7..235 /label=RSV LTR 5674..5908 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6483..6938) /label=f1 ori LTR 236..416 /label=5'LTR polyA_signal 5980..6110 /label=SV40 pA misc_feature 6111..6324 /label=SV40 ori misc_binding 418..435 /label=PBS promoter 2237..2632 /label=human EF1a promoter intron 2665..3607 /label=intron promoter complement(1872..2100) /label=H1 CDS 3651..4985 /label=GFP-Puro stem_loop complement(1824..1871) /label=shRNA BASE COUNT 2170 a 2389 c 2444 g 2107 t 38 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 1861 nnnnnnnnnn ntttttagag tggtctcata cagaacttat aagattccca aatccaaaga 1921 catttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc 1981 cactcccctg tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg 2041 cctagtgaca ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc 2101 ggccggatcc aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa 2161 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat 2221 tcaaaatttt atcgatgcct ccccgtcacc acccccccca acccgccccg accggagctg 2281 agagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga ccgtcgttaa 2341 actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc cgcccgctct 2401 cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca gtgggcagag 2461 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc 2521 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 2581 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 2641 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 2701 tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt 2761 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 2821 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 2881 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 2941 aaatttttga tgatatcctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 3001 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 3061 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 3121 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 3181 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 3241 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 3301 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 3361 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 3421 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 3481 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 3541 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 3601 atttcaggtg tcgtgaaaac tacccctcta gagatccacc ggtcgccacc atggtgagca 3661 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 3721 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 3781 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 3841 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 3901 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 3961 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 4021 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 4081 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 4141 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 4201 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 4261 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 4321 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggac ccaccggtcg 4381 ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 4441 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 4501 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 4561 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 4621 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 4681 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 4741 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 4801 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 4861 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 4921 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 4981 cctgagcggc cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 5041 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 5101 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 5161 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 5221 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 5281 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 5341 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 5401 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 5461 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 5521 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 5581 ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta 5641 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 5701 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 5761 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 5821 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 5881 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 5941 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 6001 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 6061 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 6121 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 6181 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 6241 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta 6301 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa 6361 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 6421 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 6481 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 6541 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 6601 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 6661 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 6721 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 6781 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 6841 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 6901 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 6961 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 7021 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 7081 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 7141 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 7201 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 7261 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 7321 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 7381 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 7441 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 7501 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 7561 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 7621 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 7681 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 7741 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 7801 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 7861 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 7921 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 7981 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 8041 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 8101 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 8161 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 8221 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 8281 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 8341 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 8401 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 8461 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 8521 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 8581 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 8641 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 8701 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 8761 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 8821 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 8881 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 8941 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 9001 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 9061 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct 9121 cactaaaggg aacaaaagct ggagctgc //