LOCUS pLV-mU6-EF1a-RFP-Bsd 9033 bp DNA circular 20-DEC-2009 COMMENT COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 6954..7814 /label=Amp(R) promoter 8015..8555 /label=pUC ori promoter 7..235 /label=RSV LTR 5559..5793 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6368..6823) /label=f1 ori LTR 236..416 /label=5' LTR polyA_signal 5865..5995 /label=SV40 pA misc_feature 5996..6209 /label=SV40 ori promoter 2329..2724 /label=human EF1a promoter intron 2757..3699 /label=intron promoter complement(1872..2186) /label=mouse U6 promoter CDS 3746..4870 /label=RFP-Bsd stem_loop 1824..1871 /label=shRNA BASE COUNT 2203 a 2236 c 2388 g 2168 t 38 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 1861 nnnnnnnnnn ntttttaggc ttttctccaa gggatattta tagtctcaaa acacacaatt 1921 actttacagt tagggtgagt ttccttttgt gctgtttttt aaaataataa tttagtattt 1981 gtatctctta tagaaatcca agcctatcat gtaaaatgta gctagtatta aaaagaacag 2041 attatctgtc ttttatcgca cattaagcct ctatagttac taggaaatat tatatgcaaa 2101 ttaaccgggg caggggagta gccgagcttc tcccacaagt ctgtgcgagg gggccggcgc 2161 gggcctagag atggcggcgt cggatcgaat tcggccggat ccaactttta aaagaaaagg 2221 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 2281 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgatgc ctccccgtca 2341 ccaccccccc caacccgccc cgaccggagc tgagagtaat tcatacaaaa ggactcgccc 2401 ctgccttggg gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc 2461 gctgcgttcc cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg 2521 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt 2581 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg 2641 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa 2701 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa 2761 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt 2821 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga 2881 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt 2941 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt 3001 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgatatcc tgcgacgctt 3061 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt 3121 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg 3181 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct 3241 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg 3301 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca 3361 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg 3421 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg 3481 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt 3541 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac 3601 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag 3661 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaaa actacccctc 3721 tagagtcgag ctaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat 3781 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt 3841 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 3901 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta 3961 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt 4021 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt 4081 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac 4141 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc 4201 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct 4261 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt 4321 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga 4381 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 4441 cgagctgtac aaggacccac cggtcgccac catggccaag cctttgtctc aagaagaatc 4501 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag 4561 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca 4621 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc 4681 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg 4741 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga 4801 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg 4861 ggagggctaa gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg 4921 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 4981 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 5041 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 5101 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 5161 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 5221 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 5281 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 5341 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 5401 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 5461 gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag 5521 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 5581 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag 5641 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 5701 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 5761 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc 5821 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag 5881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 5941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct 6001 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 6061 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6121 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg 6181 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg 6241 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 6301 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 6361 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 6421 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 6481 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 6541 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 6601 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 6661 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 6721 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 6781 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg 6841 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 6901 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 6961 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 7021 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 7081 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 7141 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 7201 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 7261 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 7321 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 7381 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 7441 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 7501 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 7561 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 7621 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 7681 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 7741 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 7801 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 7861 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 7921 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 7981 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 8041 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 8101 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 8161 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 8221 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 8281 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 8341 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 8401 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 8461 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 8521 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 8581 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 8641 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 8701 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 8761 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 8821 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 8881 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 8941 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta 9001 accctcacta aagggaacaa aagctggagc tgc //