LOCUS pLV-H1-EF1a-GFP-Bsd 8947 bp DNA circular 20-DEC-2009 COMMENT COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 6868..7728 /label=Amp(R) promoter 7929..8469 /label=pUC ori promoter 7..235 /label=RSV LTR 5473..5707 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6282..6737) /label=f1 ori LTR 236..416 /label=5' LTR polyA_signal 5779..5909 /label=SV40 pA misc_feature 5910..6123 /label=SV40 ori promoter 2237..2632 /label=human EF1a promoter intron 2665..3607 /label=intron promoter complement(1872..2100) /label=H1 promoter CDS 3651..4784 /label=GFP-Bsd stem_loop 1824..1871 /label=shRNA BASE COUNT 2172 a 2260 c 2354 g 2123 t 38 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnttggatcc aannnnnnnn 1861 nnnnnnnnnn ntttttagag tggtctcata cagaacttat aagattccca aatccaaaga 1921 catttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc 1981 cactcccctg tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg 2041 cctagtgaca ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc 2101 ggccggatcc aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa 2161 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat 2221 tcaaaatttt atcgatgcct ccccgtcacc acccccccca acccgccccg accggagctg 2281 agagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga ccgtcgttaa 2341 actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc cgcccgctct 2401 cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca gtgggcagag 2461 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc 2521 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 2581 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 2641 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 2701 tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt 2761 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 2821 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 2881 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 2941 aaatttttga tgatatcctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 3001 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 3061 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 3121 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 3181 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 3241 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 3301 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 3361 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 3421 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 3481 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 3541 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 3601 atttcaggtg tcgtgaaaac tacccctcta gagatccacc ggtcgccacc atggtgagca 3661 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 3721 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 3781 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 3841 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 3901 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 3961 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 4021 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 4081 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 4141 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 4201 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 4261 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 4321 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggac ccaccggtcg 4381 ccaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta 4441 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg 4501 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac 4561 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga 4621 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg 4681 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg 4741 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcggcc gcgtcgacaa 4801 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 4861 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 4921 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 4981 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 5041 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 5101 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 5161 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 5221 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 5281 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 5341 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc 5401 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 5461 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct 5521 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 5581 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 5641 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 5701 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 5761 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 5821 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 5881 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 5941 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 6001 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 6061 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg 6121 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 6181 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 6241 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 6301 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 6361 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 6421 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 6481 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 6541 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 6601 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 6661 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 6721 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 6781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 6841 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 6901 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 6961 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 7021 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 7081 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 7141 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 7201 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 7261 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 7321 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 7381 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 7441 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 7501 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 7561 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 7621 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 7681 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 7741 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 7801 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 7861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 7921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 7981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 8041 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 8101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 8161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 8221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 8281 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 8341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 8401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 8461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 8521 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 8581 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 8641 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 8701 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 8761 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 8821 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 8881 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg 8941 gagctgc //