LOCUS pLV-hU6-EF1a-Bsd 8257 bp DNA circular 31-JUL-2008 FEATURES Location/Qualifiers CDS 6178..7038 /gene=Amp(R) promoter 7239..7779 /gene=pUC ori promoter 7..235 /gene=RSV LTR 4783..5017 /gene=3'LTR intron 526..1690 /gene=Intron rep_origin complement(5592..6047) /gene=f1 ori LTR 236..416 /gene=5'LTR polyA_signal 5089..5219 /gene=SV40 pA misc_feature 5220..5433 /gene=SV40 ori promoter 2282..2677 /gene=human EF1a promoter intron 2710..3652 /gene=intron stem_loop 1824..1875 /gene=shRNA promoter complement(1876..2139) /gene=human U6 promoter CDS 3696..4094 /gene=Bsd(R) BASE COUNT 2035 a 1997 c 2127 g 2056 t 42 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 1861 nnnnnnnnnn nnnnnttttt ttcgtccttt ccacaagata tataaagcca agaaatcgaa 1921 atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc 1981 aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt 2041 tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga 2101 aggaatcatg ggaaataggc cctcttcctg cccgaccttg aattcggccg gatccaactt 2161 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 2221 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga 2281 tgcctccccg tcaccacccc ccccaacccg ccccgaccgg agctgagagt aattcataca 2341 aaaggactcg cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta 2401 gaacccagag atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt 2461 ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 2521 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc 2581 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 2641 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 2701 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc 2761 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga 2821 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc 2881 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca 2941 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgata 3001 tcctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac 3061 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg 3121 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc 3181 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc 3241 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc 3301 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac 3361 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg 3421 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg 3481 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag 3541 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg 3601 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg 3661 aaaactaccc ctctagagat ccaccggtcg ccaccatggc caagcctttg tctcaagaag 3721 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact 3781 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat 3841 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg 3901 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc 3961 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga 4021 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg 4081 tgtgggaggg ctaagcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag 4141 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 4201 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 4261 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 4321 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 4381 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 4441 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 4501 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 4561 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 4621 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 4681 ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag 4741 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 4801 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 4861 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 4921 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 4981 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 5041 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 5101 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 5161 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 5221 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 5281 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 5341 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa 5401 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 5461 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 5521 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 5581 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 5641 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 5701 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 5761 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 5821 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 5881 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 5941 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 6001 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 6061 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 6121 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 6181 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 6241 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 6301 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 6361 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 6421 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 6481 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 6541 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 6601 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 6661 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 6721 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 6781 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 6841 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 6901 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 6961 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 7021 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 7081 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 7141 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 7201 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 7261 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 7321 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 7381 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 7441 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 7501 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 7561 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 7621 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 7681 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 7741 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 7801 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 7861 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 7921 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 7981 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 8041 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 8101 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 8161 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca 8221 attaaccctc actaaaggga acaaaagctg gagctgc //