LOCUS pLV-mU6-EF1a-puro 8509 bp DNA circular 23-JUN-2008 SOURCE COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 6430..7290 /gene=Amp(R) /label=Amp(R) promoter 7491..8031 /gene=pUC ori /label=pUC ori promoter 7..235 /gene=RSV /label=RSV LTR 5035..5269 /gene=3'LTR /label=3'LTR intron 526..1690 /gene=Intron /label=Intron rep_origin complement(5844..6299) /gene=f1 ori /label=f1 ori LTR 236..416 /gene=5'LTR /label=5'LTR polyA_signal 5341..5471 /gene=SV40 pA /label=SV40 pA misc_feature 5472..5685 /gene=SV40 ori /label=SV40 ori promoter 2333..2728 /gene=human EF1a promoter /label=human EF1a promoter intron 2761..3703 /gene=intron /label=intron promoter complement(1876..2190) /gene=mouse U6 promoter /label=mouse U6 promoter stem_loop 1824..1875 /gene=shRNA /label=shRNA CDS 3747..4346 /gene=Puro(R) /label=Puro(R) BASE COUNT 2035 a 2130 c 2252 g 2050 t 42 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 1861 nnnnnnnnnn nnnnnttttt aggcttttct ccaagggata tttatagtct caaaacacac 1921 aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata ataatttagt 1981 atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt attaaaaaga 2041 acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa atattatatg 2101 caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc gagggggccg 2161 gcgcgggcct agagatggcg gcgtcggatc gaattcggcc ggatccaact tttaaaagaa 2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg atgcctcccc 2341 gtcaccaccc cccccaaccc gccccgaccg gagctgagag taattcatac aaaaggactc 2401 gcccctgcct tggggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga 2461 gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag 2521 catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 2581 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac 2641 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 2701 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 2761 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg 2821 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt 2881 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg 2941 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc 3001 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgat atcctgcgac 3061 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc 3121 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag 3181 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc 3241 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc 3301 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag 3361 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa 3421 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc 3481 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg 3541 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg 3601 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct 3661 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaaaactacc 3721 cctctagaga tccaccggtc gccaccatga ccgagtacaa gcccacggtg cgcctcgcca 3781 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 3841 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 3901 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 3961 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 4021 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 4081 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 4141 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 4201 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 4261 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 4321 gcatgacccg caagcccggt gcctgagcgg ccgcgtcgac aatcaacctc tggattacaa 4381 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 4441 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 4501 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 4561 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 4621 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 4681 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 4741 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 4801 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 4861 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 4921 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca 4981 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 5041 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 5101 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 5161 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 5221 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 5281 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 5341 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 5401 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 5461 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 5521 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 5581 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 5641 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 5701 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 5761 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 5821 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 5881 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 5941 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 6001 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 6061 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 6121 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 6181 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 6241 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 6301 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 6361 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 6421 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 6481 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 6541 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 6601 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 6661 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 6721 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 6781 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 6841 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 6901 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 6961 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 7021 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 7081 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 7141 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 7201 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 7261 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 7321 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 7381 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 7441 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 7501 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 7561 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 7621 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 7681 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 7741 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 7801 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 7861 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 7921 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 7981 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 8041 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 8101 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 8161 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 8221 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 8281 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 8341 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 8401 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 8461 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc //