LOCUS pLV-H1-EF1a-red 8525 bp DNA circular 23-JUN-2008 FEATURES Location/Qualifiers CDS 6446..7306 /gene=Amp(R) promoter 7507..8047 /gene=pUC ori promoter 7..235 /gene=RSV LTR 5051..5285 /gene=3'LTR intron 526..1690 /gene=Intron rep_origin complement(5860..6315) /gene=f1 ori LTR 236..416 /gene=5'LTR polyA_signal 5357..5487 /gene=SV40 pA misc_feature 5488..5701 /gene=SV40 ori promoter 2241..2636 /gene=human EF1a promoter intron 2669..3611 /gene=intron stem_loop 1824..1875 /gene=shRNA CDS 3658..4362 /gene=RFP promoter complement(1876..2104) /gene=H1 promoter BASE COUNT 2067 a 2133 c 2249 g 2034 t 42 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 1861 nnnnnnnnnn nnnnnttttt agagtggtct catacagaac ttataagatt cccaaatcca 1921 aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg 1981 gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt 2041 cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga 2101 attcggccgg atccaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2221 aaattcaaaa ttttatcgat gcctccccgt caccaccccc cccaacccgc cccgaccgga 2281 gctgagagta attcatacaa aaggactcgc ccctgccttg gggaatccca gggaccgtcg 2341 ttaaactccc actaacgtag aacccagaga tcgctgcgtt cccgccccct cacccgcccg 2401 ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc 2461 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 2521 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 2581 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 2641 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 2701 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt 2761 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 2821 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 2881 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 2941 tttaaaattt ttgatgatat cctgcgacgc tttttttctg gcaagatagt cttgtaaatg 3001 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 3061 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 3121 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 3181 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 3241 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 3301 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 3361 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 3421 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 3481 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 3541 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 3601 ttccatttca ggtgtcgtga aaactacccc tctagagtcg agctaccggt cgccaccatg 3661 gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc 3721 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 3781 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc 3841 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc 3901 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 3961 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac 4021 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc 4081 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag 4141 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc 4201 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac 4261 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc 4321 caccacctgt tcctgtacgg catggacgag ctgtacaagt aagcggccgc gtcgacaatc 4381 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 4441 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 4501 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 4561 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 4621 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 4681 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 4741 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 4801 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 4861 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 4921 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc 4981 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 5041 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg 5101 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 5161 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 5221 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 5281 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 5341 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 5401 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 5461 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc 5521 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 5581 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 5641 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct 5701 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 5761 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 5821 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat 5881 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 5941 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 6001 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 6061 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 6121 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 6181 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 6241 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 6301 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 6361 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 6421 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 6481 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 6541 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 6601 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 6661 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 6721 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 6781 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 6841 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 6901 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 6961 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 7021 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 7081 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 7141 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 7201 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 7261 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 7321 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 7381 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 7441 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 7501 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 7561 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 7621 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 7681 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 7741 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 7801 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 7861 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 7921 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 7981 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 8041 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 8101 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 8161 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 8221 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 8281 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 8341 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 8401 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 8461 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga 8521 gctgc //