LOCUS pLV-hU6-EF1a-green 8579 bp DNA circular 23-JUN-2008 FEATURES Location/Qualifiers CDS 6500..7360 /gene=Amp(R) promoter 7561..8101 /gene=pUC ori promoter 7..235 /gene=RSV LTR 5105..5339 /gene=3'LTR intron 526..1690 /gene=Intron rep_origin complement(5914..6369) /gene=f1 ori LTR 236..416 /gene=5'LTR polyA_signal 5411..5541 /gene=SV40 pA misc_feature 5542..5755 /gene=SV40 ori promoter 2282..2677 /gene=human EF1a promoter intron 2710..3652 /gene=intron CDS 3696..4415 /gene=GFP stem_loop 1824..1875 /gene=shRNA promoter complement(1876..2139) /gene=human U6 promoter BASE COUNT 2121 a 2132 c 2216 g 2068 t 42 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaaca ctagtaaaaa aaannnnnnn nnnnnnnnnn nnnnttggat ccaannnnnn 1861 nnnnnnnnnn nnnnnttttt ttcgtccttt ccacaagata tataaagcca agaaatcgaa 1921 atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc 1981 aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt 2041 tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga 2101 aggaatcatg ggaaataggc cctcttcctg cccgaccttg aattcggccg gatccaactt 2161 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 2221 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga 2281 tgcctccccg tcaccacccc ccccaacccg ccccgaccgg agctgagagt aattcataca 2341 aaaggactcg cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta 2401 gaacccagag atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt 2461 ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 2521 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc 2581 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 2641 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 2701 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc 2761 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga 2821 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc 2881 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca 2941 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgata 3001 tcctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac 3061 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg 3121 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc 3181 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc 3241 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc 3301 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac 3361 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg 3421 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg 3481 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag 3541 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg 3601 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg 3661 aaaactaccc ctctagagat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt 3721 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 3781 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 3841 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 3901 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 3961 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 4021 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 4081 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 4141 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 4201 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 4261 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 4321 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 4381 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgtcgac aatcaacctc 4441 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 4501 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 4561 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 4621 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 4681 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 4741 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 4801 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 4861 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 4921 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 4981 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc 5041 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 5101 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 5161 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 5221 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 5281 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 5341 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 5401 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 5461 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 5521 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc 5581 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 5641 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 5701 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg 5761 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 5821 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 5881 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg 5941 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 6001 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 6061 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 6121 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 6181 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 6241 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 6301 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 6361 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 6421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 6481 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 6541 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 6601 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 6661 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 6721 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 6781 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 6841 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 6901 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 6961 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 7021 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 7081 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 7141 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 7201 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 7261 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 7321 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 7381 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 7441 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 7501 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 7561 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 7621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 7681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 7741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 7801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 7861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 7921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 7981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 8041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 8101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 8161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 8221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 8281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 8341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 8401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 8461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 8521 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc //