LOCUS pLV-miR-Locker 9088 bp DNA circular 24-AUG-2010 SOURCE COMMENT COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers misc_feature 1807..1929 /label=cPPT CDS 7009..7869 /label=Amp(R) promoter 8070..8610 /label=pUC ori promoter 7..235 /label=RSV LTR 5614..5848 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6423..6878) /label=f1 ori LTR 236..416 /label=5'LTR polyA_signal 5920..6050 /label=SV40 pA misc_feature 6051..6264 /label=SV40 ori misc_feature 4490..5086 /label=WPRE promoter 1933..2328 /label=human EF1a promoter intron 2361..3303 /label=intron CDS 3347..4480 /label=GFP-Bsd promoter 5109..5423 /label=mU6 promoter misc_feature 5449..5498 /label=mir-locker BASE COUNT 2215 a 2278 c 2373 g 2178 t 44 others ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagaga tccaccggtc gccaccatgg tgagcaaggg 3361 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 3421 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 3481 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 3541 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 3601 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 3661 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 3721 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 3781 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 3841 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 3901 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 3961 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 4021 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggacccac cggtcgccac 4081 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat 4141 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg 4201 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt 4261 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg 4321 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct 4381 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat 4441 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcggccgcgt cgacaatcaa 4501 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 4561 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 4621 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 4681 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 4741 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 4801 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 4861 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 4921 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 4981 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 5041 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctcgagtct 5101 gaggatccga tccgacgccg ccatctctag gcccgcgccg gccccctcgc acagacttgt 5161 gggagaagct cggctactcc cctgccccgg ttaatttgca tataatattt cctagtaact 5221 atagaggctt aatgtgcgat aaaagacaga taatctgttc tttttaatac tagctacatt 5281 ttacatgata ggcttggatt tctataagag atacaaatac taaattatta ttttaaaaaa 5341 cagcacaaaa ggaaactcac cctaactgta aagtaattgt gtgttttgag actataaata 5401 tcccttggag aaaagccttg tttgtgctcg cttcggcgag cactctagnn nnnnnnnnnn 5461 nnnnnnnnnn ggatccnnnn nnnnnnnnnn nnnnnnnnag cttggagcgg acttcggtcc 5521 gctccttttt tttactagtc aacggtacct ttaagaccaa tgacttacaa ggcagctgta 5581 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 5641 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 5701 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 5761 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 5821 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 5881 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 5941 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 6001 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 6061 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 6121 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 6181 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta 6241 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa 6301 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 6361 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 6421 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 6481 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 6541 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 6601 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 6661 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 6721 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 6781 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 6841 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 6901 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 6961 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 7021 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 7081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 7141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 7201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 7261 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 7321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 7381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 7441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 7501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 7561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 7621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 7681 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 7741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 7801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 7861 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 7921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 7981 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 8041 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 8101 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 8161 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 8221 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 8281 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 8341 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 8401 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 8461 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 8521 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 8581 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 8641 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 8701 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 8761 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 8821 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 8881 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 8941 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 9001 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct 9061 cactaaaggg aacaaaagct ggagctgc //