LOCUS pLV-EF1a-MCS-IRES-GFP-Puro 9506 bp DNA circular 7-OCT-2010 SOURCE COMMENT COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7427..8287 /label=Amp(R) promoter 8488..9028 /label=pUC ori promoter 7..235 /label=RSV LTR 6032..6266 /label=3' LTR intron 526..1690 /label=Intron rep_origin 6841..7296 /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6469..6682 /label=SV40 ori misc_feature 5353..5949 /label=WPRE promoter 1933..2328 /label=human EF1a promoter intron 2361..3303 /label=intron RBS 3401..3985 /label=IRES CDS 4009..5343 /label=GFP-Puro BASE COUNT 2259 a 2495 c 2554 g 2198 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 3961 aaaacacgat gataatatgg ccacactaga gatccaccgg tcgccaccat ggtgagcaag 4021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 4081 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 4141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 4201 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 4261 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 4321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 4381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 4441 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 4501 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 4561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 4621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 4681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggaccc accggtcgcc 4741 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 4801 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 4861 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 4921 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 4981 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 5041 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 5101 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 5161 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 5221 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 5281 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 5341 tgagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 5401 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 5461 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 5521 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 5581 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 5641 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 5701 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 5761 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 5821 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 5881 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 5941 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga 6001 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 6061 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6121 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6181 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6241 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6301 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6361 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6421 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc 6481 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 6541 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 6601 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata 6661 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 6721 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 6781 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg 6841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 6901 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 6961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 7021 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 7081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 7141 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 7201 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 7261 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt 7321 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 7381 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 7441 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 7501 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 7561 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 7621 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 7681 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 7741 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 7801 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 7861 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 7921 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 7981 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 8041 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 8101 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 8161 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 8221 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 8281 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 8341 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 8401 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 8461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 8521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 8581 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 8641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 8701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 8761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 8821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 8881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 8941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 9001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 9061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 9121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 9181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 9241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 9301 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 9361 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 9421 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca 9481 ctaaagggaa caaaagctgg agctgc //