LOCUS pLV-EF1a-MCS-IRES-RFP-Puro 9500 bp DNA circular 15-AUG-2009 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7421..8281 /label=Amp(R) promoter 8482..9022 /label=pUC ori promoter 7..235 /label=RSV LTR 6026..6260 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6835..7290) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6463..6676 /label=SV40 ori misc_feature 5347..5943 /label=WPRE promoter 1933..2327 /label=human EF1a promoter intron 2361..3303 /label=intron CDS 4012..5337 /label=RFP-Puro RBS 3401..3985 /label=IRES BASE COUNT 2250 a 2480 c 2573 g 2197 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 3961 aaaacacgat gataatatgg ccacactaga gtcgagctac cggtcgccac catggtgagc 4021 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg 4081 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 4141 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg 4201 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac 4261 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 4321 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc 4381 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag 4441 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag 4501 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 4561 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 4621 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 4681 ggccgccact ccaccggcgg catggacgag ctgtacaagg acccaccggt cgccaccatg 4741 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 4801 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 4861 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 4921 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 4981 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5041 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5101 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5161 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5221 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5281 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagcg 5341 gccgcgtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 5401 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 5461 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 5521 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 5581 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 5641 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 5701 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 5761 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 5821 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 5881 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 5941 ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 6001 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga 6061 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 6121 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 6181 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 6241 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 6301 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 6361 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 6421 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc 6481 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 6541 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 6601 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt 6661 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 6721 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 6781 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc 6841 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 6901 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 6961 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 7021 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 7081 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 7141 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 7201 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 7261 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg 7321 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 7381 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 7441 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 7501 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 7561 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 7621 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 7681 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 7741 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 7801 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 7861 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 7921 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 7981 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 8041 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 8101 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 8161 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 8221 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 8281 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 8341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 8401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 8461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 8521 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 8581 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 8641 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 8701 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 8761 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 8821 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 8881 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 8941 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 9001 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 9061 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 9121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 9181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 9241 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 9301 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 9361 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 9421 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag 9481 ggaacaaaag ctggagctgc //