LOCUS pLV-EF1a-MCS-IRES-RFP 8884 bp DNA circular 22-MAY-2007 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 6805..7665 /label=Amp(R) promoter 7866..8406 /label=pUC ori promoter 7..235 /label=RSV LTR 5410..5644 /label=3' LTR intron 526..1690 /label=Intron rep_origin 6219..6674 /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 5847..6060 /label=SV40 ori misc_feature 4731..5327 /label=WPRE promoter 1933..2327 /label=human EF1a promoter intron 2361..3303 /label=intron RBS 3401..3984 /label=IRES CDS 4011..4721 /label=RFP BASE COUNT 2162 a 2235 c 2365 g 2122 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat 3481 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct 3541 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg 3601 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc 3661 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg 3721 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg 3781 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga 3841 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt 3901 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa 3961 aaacacgatg ataatatggc cacactagag tcgagctacc ggtcgccacc atggtgagca 4021 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg 4081 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 4141 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg 4201 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca 4261 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 4321 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca 4381 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga 4441 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg 4501 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga 4561 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt 4621 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg 4681 gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgcg tcgacaatca 4741 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 4801 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 4861 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 4921 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 4981 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 5041 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 5101 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 5161 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 5221 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 5281 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg 5341 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 5401 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt 5461 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 5521 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 5581 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 5641 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 5701 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 5761 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 5821 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc 5881 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 5941 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 6001 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc 6061 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 6121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 6181 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt 6241 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 6301 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 6361 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 6421 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 6481 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 6541 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 6601 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 6661 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 6721 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 6781 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 6841 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 6901 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 6961 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 7021 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 7081 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 7141 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 7201 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 7261 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 7321 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 7381 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 7441 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 7501 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 7561 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 7621 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 7681 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 7741 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 7801 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 7861 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 7921 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 7981 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 8041 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 8101 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 8161 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 8221 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 8281 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 8341 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 8401 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 8461 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 8521 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 8581 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 8641 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 8701 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 8761 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc 8821 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag 8881 ctgc //