LOCUS pLV-EF1a-MCS-IRES-GFP 8892 bp DNA circular 22-MAY-2007 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 6813..7673 /label=Amp(R) promoter 7874..8414 /label=pUC ori promoter 7..235 /label=RSV LTR 5418..5652 /label=3' LTR intron 526..1690 /label=Intron rep_origin 6227..6682 /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 5855..6068 /label=SV40 ori misc_feature 4739..5335 /label=WPRE promoter 1933..2327 /label=human EF1a promoter intron 2361..3303 /label=intron CDS 4009..4728 /label=EGFP RBS 3401..3985 /label=IRES BASE COUNT 2172 a 2251 c 2346 g 2123 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 3961 aaaacacgat gataatatgg ccacactaga gatccaccgg tcgccaccat ggtgagcaag 4021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 4081 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 4141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 4201 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 4261 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 4321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 4381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 4441 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 4501 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 4561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 4621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 4681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgtc 4741 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 4801 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 4861 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 4921 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 4981 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 5041 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 5101 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg 5161 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 5221 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 5281 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt 5341 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 5401 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt 5461 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 5521 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 5581 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 5641 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 5701 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 5761 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 5821 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 5881 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 5941 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 6001 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac 6061 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 6121 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 6181 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc 6241 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 6301 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 6361 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 6421 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 6481 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 6541 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 6601 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 6661 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta 6721 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 6781 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 6841 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 6901 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 6961 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 7021 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 7081 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 7141 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 7201 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 7261 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 7321 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 7381 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 7441 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 7501 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 7561 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 7621 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 7681 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 7741 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 7801 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 7861 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 7921 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 7981 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 8041 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 8101 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 8161 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 8221 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 8281 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 8341 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 8401 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 8461 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 8521 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 8581 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 8641 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 8701 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 8761 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 8821 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa 8881 agctggagct gc //