LOCUS pLV-EF1a-MCS-IRES-Neo 8954 bp DNA circular 4-FEB-2008 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 6875..7735 /label=Amp(R) promoter 7936..8476 /label=pUC ori promoter 7..235 /label=RSV LTR 5480..5714 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6289..6744) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 5917..6130 /label=SV40 ori misc_feature 4801..5397 /label=WPRE promoter 1933..2327 /label=human EF1a promoter intron 2361..3303 /label=intron CDS 3997..4791 /label=Neo RBS 3401..3985 /label=IRES BASE COUNT 2139 a 2231 c 2389 g 2195 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 3961 aaaacacgat gataatatgg ccacactaga gccaccatga ttgaacaaga tggattgcac 4021 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 4081 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 4141 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 4201 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 4261 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 4321 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 4381 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 4441 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 4501 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 4561 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 4621 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 4681 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 4741 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcggccgcg 4801 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 4861 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 4921 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 4981 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 5041 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 5101 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 5161 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 5221 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 5281 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 5341 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 5401 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 5461 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct 5521 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 5581 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 5641 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 5701 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 5761 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 5821 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 5881 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 5941 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 6001 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 6061 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt 6121 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 6181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 6241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta 6301 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 6361 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 6421 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 6481 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 6541 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 6601 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 6661 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 6721 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc 6781 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 6841 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 6901 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 6961 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 7021 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 7081 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 7141 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 7201 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 7261 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 7321 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 7381 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 7441 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 7501 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 7561 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 7621 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 7681 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 7741 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 7801 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 7861 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 7921 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 7981 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 8041 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 8101 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 8161 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 8221 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 8281 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 8341 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 8401 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 8461 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 8521 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 8581 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 8641 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct 8701 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 8761 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 8821 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 8881 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca 8941 aaagctggag ctgc //