LOCUS pLV-EF1a-MCS-IRES-Bsd 8570 bp DNA circular 30-JAN-2008 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 6491..7351 /label=Amp(R) promoter 7552..8092 /label=pUC ori promoter 7..235 /label=RSV LTR 5096..5330 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(5905..6360) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 5533..5746 /label=SV40 ori misc_feature 4417..5013 /label=WPRE promoter 1933..2327 /label=human EF1a promoter intron 2361..3303 /label=intron CDS 4009..4407 /label=Bsd RBS 3401..3985 /label=IRES BASE COUNT 2086 a 2116 c 2257 g 2111 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 3961 aaaacacgat gataatatgg ccacactaga gatccaccgg tcgccaccat ggccaagcct 4021 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc 4081 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact 4141 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact 4201 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg 4261 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc 4321 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg 4381 ccctctggtt atgtgtggga gggctaagcg gccgcgtcga caatcaacct ctggattaca 4441 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 4501 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 4561 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 4621 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 4681 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 4741 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 4801 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 4861 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 4921 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 4981 gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc 5041 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 5101 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg 5161 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 5221 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 5281 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 5341 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 5401 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 5461 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 5521 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg 5581 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 5641 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 5701 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt 5761 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 5821 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 5881 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg 5941 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 6001 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 6061 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 6121 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 6181 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 6241 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 6301 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt 6361 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 6421 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 6481 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 6541 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 6601 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 6661 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 6721 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 6781 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 6841 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 6901 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 6961 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 7021 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 7081 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 7141 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 7201 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 7261 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 7321 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 7381 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 7441 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 7501 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 7561 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 7621 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 7681 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 7741 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 7801 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 7861 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 7921 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 7981 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 8041 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 8101 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 8161 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 8221 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 8281 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 8341 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 8401 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 8461 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 8521 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc //