LOCUS pLV-EF1a-MCS-IRES-Hyg 9197 bp DNA circular 30-JAN-2008 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7118..7978 /label=Amp(R) promoter 8179..8719 /label=pUC ori promoter 7..235 /label=RSV LTR 5723..5957 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6532..6987) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6160..6373 /label=SV40 ori misc_feature 5044..5640 /label=WPRE promoter 1933..2327 /label=human EF1a promoter intron 2361..3303 /label=intron CDS 4009..5034 /label=Hyg RBS 3401..3985 /label=IRES BASE COUNT 2208 a 2293 c 2457 g 2239 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 3961 aaaacacgat gataatatgg ccacactaga gatccaccgg tcgccaccat gaaaaagcct 4021 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac 4081 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt 4141 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat 4201 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc 4261 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct 4321 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg 4381 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac 4441 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact 4501 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg 4561 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc 4621 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat 4681 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag 4741 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg 4801 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat 4861 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc 4921 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta 4981 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga ataggcggcc 5041 gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 5101 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 5161 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 5221 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 5281 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 5341 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 5401 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 5461 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 5521 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 5581 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 5641 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 5701 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct 5761 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 5821 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 5881 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 5941 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 6001 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 6061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 6121 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 6181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 6241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 6301 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt 6361 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 6421 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 6481 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct 6541 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 6601 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 6661 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 6721 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 6781 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 6841 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 6901 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 6961 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac 7021 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 7081 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 7141 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 7201 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 7261 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 7321 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 7381 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 7441 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 7501 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 7561 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 7621 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 7681 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 7741 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 7801 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 7861 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 7921 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 7981 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 8041 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 8101 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 8161 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 8221 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 8281 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 8341 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 8401 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 8461 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 8521 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 8581 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 8641 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 8701 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 8761 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 8821 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 8881 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 8941 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 9001 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 9061 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 9121 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga 9181 acaaaagctg gagctgc //