LOCUS pLV-EF1a-MCS-IRES-Puro 8771 bp DNA circular 19-MAR-1980 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 6692..7552 /label=Amp(R) promoter 7753..8293 /label=pUC ori promoter 7..235 /label=RSV LTR 5297..5531 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6106..6561) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 5734..5947 /label=SV40 ori promoter 1933..2327 /label=human EF1a promoter intron 2361..3303 /label=intron CDS 4009..4608 /label=Puro RBS 3401..3985 /label=EMCV IRES misc_feature 4618..5214 /label=WPRE BASE COUNT 2084 a 2245 c 2347 g 2095 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 3961 aaaacacgat gataatatgg ccacactaga gatccaccgg tcgccaccat gaccgagtac 4021 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 4081 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 4141 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 4201 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 4261 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 4321 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 4381 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 4441 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 4501 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 4561 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc ggccgcgtcg 4621 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 4681 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 4741 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 4801 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 4861 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 4921 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 4981 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 5041 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 5101 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 5161 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc 5221 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 5281 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt 5341 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 5401 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 5461 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 5521 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 5581 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 5641 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 5701 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg 5761 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 5821 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 5881 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg 5941 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 6001 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 6061 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg 6121 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 6181 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 6241 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 6301 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 6361 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 6421 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 6481 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 6541 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 6601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 6661 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 6721 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 6781 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 6841 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 6901 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 6961 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 7021 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 7081 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 7141 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 7201 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 7261 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 7321 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 7381 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 7441 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 7501 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 7561 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 7621 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 7681 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 7741 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 7801 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 7861 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 7921 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 7981 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 8041 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 8101 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 8161 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 8221 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 8281 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 8341 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 8401 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 8461 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 8521 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 8581 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 8641 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 8701 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 8761 gctggagctg c //