LOCUS pLV-EF1a-MCS-IRES-RFP-Neo 9695 bp DNA circular 15-AUG-2009 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7616..8476 /label=Amp(R) promoter 8677..9217 /label=pUC ori promoter 7..235 /label=RSV LTR 6221..6455 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(7030..7485) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6658..6871 /label=SV40 ori misc_feature 5542..6138 /label=WPRE promoter 1933..2327 /label=human EF1a promoter intron 2361..3303 /label=intron CDS 4012..5532 /label=RFP-Neo RBS 3401..3985 /label=IRES BASE COUNT 2307 a 2471 c 2618 g 2299 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 3961 aaaacacgat gataatatgg ccacactaga gtcgagctac cggtcgccac catggtgagc 4021 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg 4081 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 4141 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg 4201 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac 4261 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 4321 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc 4381 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag 4441 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag 4501 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 4561 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 4621 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 4681 ggccgccact ccaccggcgg catggacgag ctgtacaagg acccaccggt cgccaccatg 4741 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 4801 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 4861 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 4921 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 4981 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 5041 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 5101 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 5161 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 5221 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 5281 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 5341 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 5401 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 5461 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 5521 gagttcttct gagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat 5581 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 5641 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 5701 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 5761 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 5821 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 5881 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 5941 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 6001 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 6061 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 6121 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc 6181 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 6241 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 6301 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6361 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6421 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6481 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 6541 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 6601 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 6661 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 6721 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 6781 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt 6841 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact 6901 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 6961 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 7021 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 7081 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 7141 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 7201 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 7261 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 7321 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 7381 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 7441 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg 7501 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 7561 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 7621 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 7681 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 7741 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 7801 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 7861 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 7921 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 7981 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 8041 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 8101 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 8161 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 8221 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 8281 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 8341 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 8401 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 8461 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 8521 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 8581 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 8641 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 8701 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 8761 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 8821 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 8881 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 8941 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 9001 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 9061 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 9121 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 9181 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 9241 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 9301 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 9361 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 9421 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 9481 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 9541 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 9601 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat 9661 taaccctcac taaagggaac aaaagctgga gctgc //