LOCUS pLV-EF1a-MCS-IRES-RFP-Bsd 9299 bp DNA circular 15-AUG-2009 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7220..8080 /label=Amp(R) promoter 8281..8821 /label=pUC ori promoter 7..235 /label=RSV LTR 5825..6059 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6634..7089) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6262..6475 /label=SV40 ori misc_feature 5146..5742 /label=WPRE promoter 1933..2327 /label=human EF1a promoter intron 2361..3303 /label=intron RBS 3401..3985 /label=IRES CDS 4012..5136 /label=RFP-Bsd BASE COUNT 2252 a 2351 c 2483 g 2213 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 3961 aaaacacgat gataatatgg ccacactaga gtcgagctac cggtcgccac catggtgagc 4021 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg 4081 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 4141 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg 4201 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac 4261 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 4321 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc 4381 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag 4441 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag 4501 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 4561 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 4621 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 4681 ggccgccact ccaccggcgg catggacgag ctgtacaagg acccaccggt cgccaccatg 4741 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac 4801 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc 4861 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg 4921 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat 4981 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat 5041 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt 5101 gaattgctgc cctctggtta tgtgtgggag ggctaagcgg ccgcgtcgac aatcaacctc 5161 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 5221 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 5281 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 5341 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 5401 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 5461 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 5521 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 5581 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 5641 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 5701 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc 5761 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 5821 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 5881 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 5941 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 6001 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 6061 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 6121 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 6181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 6241 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc 6301 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 6361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 6421 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg 6481 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 6541 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 6601 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg 6661 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 6721 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 6781 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 6841 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 6901 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 6961 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 7021 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 7081 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 7141 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 7201 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 7261 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 7321 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 7381 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 7441 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 7501 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 7561 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 7621 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 7681 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 7741 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 7801 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 7861 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 7921 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 7981 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 8041 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 8101 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 8161 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 8221 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 8281 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 8341 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 8401 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 8461 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 8521 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 8581 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 8641 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 8701 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 8761 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 8821 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 8881 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 8941 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 9001 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 9061 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 9121 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 9181 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 9241 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc //