LOCUS pLV-EF1a-MCS-IRES-GFP-Bsd 9305 bp DNA circular 15-AUG-2009 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7226..8086 /label=Amp(R) promoter 8287..8827 /label=pUC ori promoter 7..235 /label=RSV LTR 5831..6065 /label=3' LTR intron 526..1690 /label=Intron rep_origin 6640..7095 /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6268..6481 /label=SV40 ori misc_feature 5152..5748 /label=WPRE promoter 1933..2327 /label=human EF1a promoter intron 2361..3303 /label=intron RBS 3401..3985 /label=IRES CDS 4009..5142 /label=GFP-Bsd BASE COUNT 2261 a 2366 c 2464 g 2214 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 3961 aaaacacgat gataatatgg ccacactaga gatccaccgg tcgccaccat ggtgagcaag 4021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 4081 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 4141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 4201 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 4261 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 4321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 4381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 4441 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 4501 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 4561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 4621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 4681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggaccc accggtcgcc 4741 accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca 4801 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac 4861 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc 4921 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc 4981 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat 5041 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg 5101 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagcggccgc gtcgacaatc 5161 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 5221 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 5281 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 5341 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 5401 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 5461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 5521 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 5581 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 5641 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 5701 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc 5761 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 5821 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg 5881 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 5941 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 6001 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 6061 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 6121 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 6181 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 6241 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc 6301 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 6361 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 6421 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct 6481 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 6541 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 6601 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat 6661 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 6721 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 6781 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 6841 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 6901 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 6961 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 7021 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 7081 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 7141 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 7201 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 7261 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 7321 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 7381 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 7441 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 7501 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 7561 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 7621 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 7681 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 7741 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 7801 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 7861 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 7921 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 7981 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 8041 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 8101 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 8161 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 8221 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 8281 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 8341 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 8401 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 8461 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 8521 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 8581 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 8641 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 8701 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 8761 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 8821 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 8881 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 8941 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 9001 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 9061 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 9121 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 9181 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 9241 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga 9301 gctgc //