LOCUS pLV-EF1a-hULK1-F2A-GFP 11482 bp DNA circular 24-OCT-2012 COMMENT COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 9403..10263 /label=Amp(R) rep_origin 10464..11004 /label=pUC ori promoter 7..235 /label=RSV LTR 8008..8242 /label=3' LTR intron 526..1690 /label=Intron rep_origin 8817..9272 /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 8445..8658 /label=SV40 ori misc_feature 7329..7925 /label=WPRE promoter 1933..3322 /label=human EF1a promoter intron 2361..3303 /label=intron misc_feature 6534..6599 /label=F2A CDS 6600..7319 /label=GFP CDS 3369..6518 /label=ULK1 BASE COUNT 2603 a 3261 c 3117 g 2501 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccgccaccat ggagcccggc cgcggcggca cagagaccgt gggcaagttc gagttctccc 3421 gcaaggacct gatcggccac ggcgccttcg cggtggtctt caagggccgc caccgcgaga 3481 agcacgattt ggaggtcgcc gtcaagtgca ttaacaagaa gaacctcgcc aagtctcaga 3541 cgctgctggg gaaggaaatc aaaatcctga aggaactgaa acatgaaaac atcgtggccc 3601 tgtacgactt ccaggaaatg gctaattctg tctacctggt tatggagtac tgcaacggtg 3661 gggacctggc cgactacctg cacgccatgc gcacgctgag cgaggacacc atcaggctct 3721 tcctgcagca gatcgcgggc gccatgcggc ttctgcacag caaaggcatc atccaccgcg 3781 acctgaaacc gcagaacatc ctgctgtcca accccgccgg ccgccgcgcc aaccccaaca 3841 gcatccgcgt caagatcgct gacttcggct tcgcgcggta cctccagagc aacatgatgg 3901 cggccacact ctgcggctcc cccatgtaca tggcccccga ggtcatcatg tcccagcact 3961 acgacgggaa ggcggacctg tggagcatcg gcaccatcgt ctaccagtgc ctgacgggga 4021 aggcgccctt ccaggccagc agcccccagg acctgcgcct gttctacgag aagaacaaga 4081 cgttggtccc caccatcccc cgggagacct cggccccgct gcggcagctg ctcctggccc 4141 tactgcaacg caaccacaag gaccgcatgg acttcgatga gttttttcat caccctttcc 4201 tcgatgccag cccctcggtc aggaaatccc cacccgtgcc tgtgccctcg tacccaagct 4261 cggggtccgg cagcagctcc agcagcagct ccacctccca cctggcctcc ccgccgtccc 4321 tgggcgagat gcagcagctg cagaagaccc tggcctcccc ggctgacacc gctggcttcc 4381 tgcacagctc ccgggactct ggtggcagca aggactcttc ctgtgacaca gacgacttcg 4441 tcatggtccc cgcgcagttt ccaggtgacc tggtggctga ggcgcccagt gccaaacccc 4501 cgccagacag cctgatgtgc agtgggagct cactggtggc ctctgcgggc ttggagagcc 4561 acggccggac cccatctcca tccccaccct gcagcagctc ccccagtccc tcaggccggg 4621 ctggcccgtt ctccagcagc aggtgcggcg cctctgtccc catcccagtc cccacgcagg 4681 tgcagaacta ccagcgcatt gagcgaaacc tgcagtcacc cacccagttc caaacacctc 4741 ggtcctctgc catccgcagg tcaggcagca ccagccccct gggctttgca agggccagcc 4801 cctcgccccc tgcccacgct gagcatggag gcgtcctggc caggaagatg tctctgggtg 4861 gaggccggcc ctacacgcca tctcctcaag ttggaaccat ccctgagcgg ccaggctgga 4921 gcgggacgcc ctccccacag ggagctgaga tgcggggtgg caggtcccct cgtccaggct 4981 cctctgcacc cgagcactct ccccgcactt ccgggctggg ctgccgcctg cacagcgccc 5041 ccaacctgtc tgacttgcac gtcgtccgcc ccaagctgcc caaacccccc acggaccccc 5101 tgggagctgt gttcagccca ccacaggcca gccctcccca gccgtcccac ggcctgcagt 5161 cctgccggaa cctgcggggc tcacccaagc tgcccgactt cctgcagcga aaccccctgc 5221 cccccatcct gggctccccc accaaggctg tgccctcctt tgacttcccg aagaccccca 5281 gctcccagaa cctgctggcc ctcctagccc ggcagggcgt ggtgatgacg ccccctcgaa 5341 accggacgct gcccgacctc tcggaggtgg gacccttcca tggtcagccg ttgggccctg 5401 gcctgcggcc aggcgaggac cccaagggcc cctttggccg gtctttcagc accagccgcc 5461 tcactgacct gctccttaag gcggcgtttg ggacacaagc cccggacccg ggcagcacgg 5521 agagcctgca ggagaagccc atggagatcg caccctcagc tggctttgga gggagcctgc 5581 acccaggagc ccgtgctggg ggcaccagca gcccttcccc ggtggtcttc accgtgggct 5641 ctcccccgag cgggagcacg cccccccagg gcccccgcac caggatgttc tcagcgggcc 5701 ccactggctc tgccagctct tctgcccgcc acctggtgcc tgggccctgc agcgaggccc 5761 cagcccctga gctccctgct ccaggacacg gctgcagctt tgccgacccc attactgcga 5821 acctggaggg ggctgtgacc ttcgaggccc ccgacctccc tgaggagacc ctcatggagc 5881 aagagcacac ggagatcctg cgtggcctgc gcttcacgct gctgttcgtg cagcacgtcc 5941 tggagatcgc agccctgaag ggcagcgcca gtgaggcggc ggggggccct gagtaccagc 6001 tgcaggagag tgtggtggcc gaccagatca gcctgctgag ccgagaatgg ggcttcgcgg 6061 aacagctggt gctgtacctg aaggtggccg agctactgtc ctccggcctg caaagtgcca 6121 tcgaccagat ccgggccggc aagctctgcc tgtcgtccac tgtgaagcag gtggtgcgca 6181 ggctgaatga gctgtacaag gccagcgtgg tgtcctgcca gggcctgagc ctgcggctgc 6241 agcgcttctt cctggacaag cagcggctcc tggaccgcat tcacagcatc actgccgaga 6301 ggctcatctt cagccacgct gtgcagatgg tgcagtcggc tgccctggac gagatgttcc 6361 agcaccgtga gggctgcgtc ccacgctacc acaaggccct gctgctcctg gaggggctgc 6421 agcacatgct ctcggaccag gccgacatcg agaacgtcac caagtgcaag ctgtgcattg 6481 agcggagact ctcggcgctg ctgactggca tctgtgccgc tagcggcagc ggagttaagc 6541 aaaccctgaa cttcgatctg cttaaactgg caggagacgt cgaatctaac ccaggaccca 6601 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 6661 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 6721 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 6781 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 6841 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 6901 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 6961 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 7021 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 7081 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 7141 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 7201 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 7261 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag 7321 cggccgcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 7381 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 7441 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 7501 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 7561 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 7621 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 7681 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 7741 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 7801 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 7861 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 7921 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 7981 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 8041 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 8101 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 8161 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 8221 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 8281 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 8341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 8401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 8461 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 8521 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 8581 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga 8641 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 8701 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 8761 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc 8821 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 8881 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 8941 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 9001 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 9061 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 9121 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 9181 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 9241 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 9301 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 9361 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 9421 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 9481 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 9541 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 9601 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 9661 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 9721 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 9781 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 9841 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 9901 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 9961 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 10021 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 10081 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 10141 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 10201 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 10261 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 10321 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 10381 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 10441 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 10501 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 10561 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 10621 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 10681 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 10741 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 10801 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 10861 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 10921 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 10981 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 11041 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 11101 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 11161 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 11221 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 11281 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 11341 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 11401 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 11461 agggaacaaa agctggagct gc //