LOCUS pLV-EF1a-hTwist-IRES-Hyg 9778 bp DNA circular 28-JAN-2013 COMMENT COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7699..8559 /label=Amp(R) promoter 8760..9300 /label=pUC ori promoter 7..235 /label=RSV LTR 6304..6538 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(7113..7568) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6741..6954 /label=SV40 ori misc_feature 5625..6221 /label=WPRE promoter 1933..3322 /label=human EF1a promoter CDS 4590..5615 /label=Hyg RBS 3982..4566 /label=IRES CDS 3369..3977 /label=hTwist intron 2361..3303 /label=intron misc_feature 1807..1929 /label=cPPT polyA_signal 6610..6740 /label=SV40 pA BASE COUNT 2309 a 2483 c 2687 g 2299 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccgccaccat gatgcaggac gtgtccagct cgccagtctc gccggccgac gacagcctga 3421 gcaacagcga ggaagagcca gaccggcagc agccgccgag cggcaagcgc gggggacgca 3481 agcggcgcag cagcaggcgc agcgcgggcg gcggcgcggg gcccggcgga gccgcgggtg 3541 ggggcgtcgg aggcggcgac gagccgggca gcccggccca gggcaagcgc ggcaagaagt 3601 ctgcgggctg tggcggcggc ggcggcgcgg gcggcggcgg cggcagcagc agcggcggcg 3661 ggagtccgca gtcttacgag gagctgcaga cgcagcgggt catggccaac gtgcgggagc 3721 gccagcgcac ccagtcgctg aacgaggcgt tcgccgcgct gcggaagatc atccccacgc 3781 tgccctcgga caagctgagc aagattcaga ccctcaagct ggcggccagg tacatcgact 3841 tcctctacca ggtcctccag agcgacgagc tggactccaa gatggcaagc tgcagctatg 3901 tggctcacga gcggctcagc tacgccttct cggtctggag gatggagggg gcctggtcca 3961 tgtccgcgtc ccactaggct agcccctctc cctccccccc ccctaacgtt actggccgaa 4021 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 4081 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 4141 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 4201 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 4261 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 4321 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 4381 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 4441 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 4501 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg 4561 gccacactag agatccaccg gtcgccacca tgaaaaagcc tgaactcacc gcgacgtctg 4621 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg 4681 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa 4741 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg 4801 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca 4861 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg 4921 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga 4981 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca 5041 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca 5101 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag 5161 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca 5221 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca 5281 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc 5341 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc 5401 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg 5461 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc 5521 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc 5581 gacgccccag cactcgtccg agggcaaagg aataggcggc cgcgtcgaca atcaacctct 5641 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 5701 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 5761 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 5821 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 5881 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 5941 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 6001 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 6061 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 6121 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 6181 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct 6241 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 6301 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg 6361 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 6421 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 6481 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 6541 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 6601 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 6661 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 6721 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc 6781 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 6841 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 6901 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc 6961 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 7021 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 7081 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc 7141 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 7201 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 7261 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 7321 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 7381 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 7441 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 7501 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 7561 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 7621 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 7681 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 7741 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 7801 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 7861 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 7921 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 7981 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 8041 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 8101 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 8161 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 8221 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 8281 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 8341 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 8401 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 8461 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 8521 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 8581 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 8641 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 8701 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 8761 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 8821 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 8881 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 8941 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 9001 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 9061 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 9121 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 9181 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 9241 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 9301 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 9361 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 9421 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 9481 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 9541 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 9601 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 9661 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 9721 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgc //