LOCUS       pLV-EF1a-hTERT-IRES-Puro       12142 bp    DNA     circular     28-OCT-2008
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             10063..10923
                     /label=Amp(R)
     promoter        11124..11664
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             8668..8902
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      9477..9932
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    8974..9104
                     /label=SV40 pA
     misc_feature    9105..9318
                     /label=SV40 ori
     misc_feature    7989..8585
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     RBS             6772..7356
                     /label=IRES
     CDS             3369..6767
                     /label=hTERT
     CDS             7380..7979
                     /label=Puro
BASE COUNT     2626 a      3395 c      3428 g      2693 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ctgccaccat gccgcgcgct ccccgctgcc gagccgtgcg ctccctgctg cgcagccact 
     3421 accgcgaggt gctgccgctg gccacgttcg tgcggcgcct ggggccccag ggctggcggc 
     3481 tggtgcagcg cggggacccg gcggctttcc gcgcgctggt ggcccagtgc ctggtgtgcg 
     3541 tgccctggga cgcacggccg ccccccgccg ccccctcctt ccgccaggtg tcctgcctga 
     3601 aggagctggt ggcccgagtg ctgcagaggc tgtgcgagcg cggcgcgaag aacgtgctgg 
     3661 ccttcggctt cgcgctgctg gacggggccc gcgggggccc ccccgaggcc ttcaccacca 
     3721 gcgtgcgcag ctacctgccc aacacggtga ccgacgcact gcgggggagc ggggcgtggg 
     3781 ggctgctgct gcgccgcgtg ggcgacgacg tgctggttca cctgctggca cgctgcgcgc 
     3841 tctttgtgct ggtggctccc agctgcgcct accaggtgtg cgggccgccg ctgtaccagc 
     3901 tcggcgctgc cactcaggcc cggcccccgc cacacgctag tggaccccga aggcgtctgg 
     3961 gatgcgaacg ggcctggaac catagcgtca gggaggccgg ggtccccctg ggcctgccag 
     4021 ccccgggtgc gaggaggcgc gggggcagtg ccagccgaag tctgccgttg cccaagaggc 
     4081 ccaggcgtgg cgctgcccct gagccggagc ggacgcccgt tgggcagggg tcctgggccc 
     4141 acccgggcag gacgcgtgga ccgagtgacc gtggtttctg tgtggtgtca cctgccagac 
     4201 ccgccgaaga agccacctct ttggagggtg cgctctctgg cacgcgccac tcccacccat 
     4261 ccgtgggccg ccagcaccac gcgggccccc catccacatc gcggccacca cgtccctggg 
     4321 acacgccttg tcccccggtg tacgccgaga ccaagcactt cctctactcc tcaggcgaca 
     4381 aggagcagct gcggccctcc ttcctactca gctctctgag gcccagcctg actggcgctc 
     4441 ggaggctcgt ggagaccatc tttctgggtt ccaggccctg gatgccaggg actccccgca 
     4501 ggttgccccg cctgccccag cgctactggc aaatgcggcc cctgtttctg gagctgcttg 
     4561 ggaaccacgc gcagtgcccc tacggggtgc tcctcaagac gcactgcccg ctgcgagctg 
     4621 cggtcacccc agcagccggt gtctgtgccc gggagaagcc ccagggctct gtggcggccc 
     4681 ccgaggagga ggacacagac ccccgtcgcc tggtgcagct gctccgccag cacagcagcc 
     4741 cctggcaggt gtacggcttc gtgcgggcct gcctgcgccg gctggtgccc ccaggcctct 
     4801 ggggctccag gcacaacgaa cgccgcttcc tcaggaacac caagaagttc atctccctgg 
     4861 ggaagcatgc caagctctcg ctgcaggagc tgacgtggaa gatgagcgtg cggggctgcg 
     4921 cttggctgcg caggagccca ggggttggct gtgttccggc cgcagagcac cgtctgcgtg 
     4981 aggagatcct ggccaagttc ctgcactggc tgatgagtgt gtacgtcgtc gagctgctca 
     5041 ggtctttctt ttatgtcacg gagaccacgt ttcaaaagaa caggctcttt ttctaccgga 
     5101 agagtgtctg gagcaagttg caaagcattg gaatcagaca gcacttgaag agggtgcagc 
     5161 tgcgggagct gtcggaagca gaggtcaggc agcatcggga agccaggccc gccctgctga 
     5221 cgtccagact ccgcttcatc cccaagcctg acgggctgcg gccgattgtg aacatggact 
     5281 acgtcgtggg agccagaacg ttccgcagag aaaagagggc cgagcgtctc acctcgaggg 
     5341 tgaaggcact gttcagcgtg ctcaactacg agcgggcgcg gcgccccggc ctcctgggcg 
     5401 cctctgtgct gggcctggac gatatccaca gggcctggcg caccttcgtg ctgcgtgtgc 
     5461 gggcccagga cccgccgcct gagctgtact ttgtcaaggt ggatgtgacg ggcgcgtacg 
     5521 acaccatccc ccaggacagg ctcacggagg tcatcgccag catcatcaaa ccccagaaca 
     5581 cgtactgcgt gcgtcggtat gccgtggtcc agaaggccgc ccatgggcac gtccgcaagg 
     5641 ccttcaagag ccacgtctct accttgacag acctccagcc gtacatgcga cagttcgtgg 
     5701 ctcacctgca ggagaccagc ccgctgaggg atgccgtcgt catcgagcag agctcctccc 
     5761 tgaatgaggc cagcagtggc ctcttcgacg tcttcctacg cttcatgtgc caccacgccg 
     5821 tgcgcatcag gggcaagtcc tacgtccagt gccaggggat cccgcagggc tccatcctct 
     5881 ccacgctgct ctgcagcctg tgctacggcg acatggagaa caagctgttt gcggggattc 
     5941 ggcgggacgg gctgctcctg cgtttggtgg atgatttctt gttggtgaca cctcacctca 
     6001 cccacgcgaa aaccttcctc aggaccctgg tccgaggtgt ccctgagtat ggctgcgtgg 
     6061 tgaacttgcg gaagacagtg gtgaacttcc ctgtagaaga cgaggccctg ggtggcacgg 
     6121 cttttgttca gatgccggcc cacggcctat tcccctggtg cggcctgctg ctggataccc 
     6181 ggaccctgga ggtgcagagc gactactcca gctatgcccg gacctccatc agagccagtc 
     6241 tcaccttcaa ccgcggcttc aaggctggga ggaacatgcg tcgcaaactc tttggggtct 
     6301 tgcggctgaa gtgtcacagc ctgtttctgg atttgcaggt gaacagcctc cagacggtgt 
     6361 gcaccaacat ctacaagatc ctcctgctgc aggcgtacag gtttcacgca tgtgtgctgc 
     6421 agctcccatt tcatcagcaa gtttggaaga accccacatt tttcctgcgc gtcatctctg 
     6481 acacggcctc cctctgctac tccatcctga aagccaagaa cgcagggatg tcgctggggg 
     6541 ccaagggcgc cgccggccct ctgccctccg aggccgtgca gtggctgtgc caccaagcat 
     6601 tcctgctcaa gctgactcga caccgtgtca cctacgtgcc actcctgggg tcactcagga 
     6661 cagcccagac gcagctgagt cggaagctcc cggggacgac gctgactgcc ctggaggccg 
     6721 cagccaaccc ggcactgccc tcagacttca agaccatcct ggactgagct agcccctctc 
     6781 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg 
     6841 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg 
     6901 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag 
     6961 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt 
     7021 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc 
     7081 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga 
     7141 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga 
     7201 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct 
     7261 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg 
     7321 ttttcctttg aaaaacacga tgataatatg gccacactag agatccaccg gtcgccacca 
     7381 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 
     7441 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 
     7501 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 
     7561 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 
     7621 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 
     7681 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 
     7741 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 
     7801 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 
     7861 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 
     7921 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag 
     7981 cggccgcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 
     8041 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 
     8101 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 
     8161 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 
     8221 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 
     8281 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 
     8341 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 
     8401 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 
     8461 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 
     8521 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 
     8581 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 
     8641 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 
     8701 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 
     8761 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 
     8821 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 
     8881 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 
     8941 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 
     9001 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     9061 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 
     9121 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 
     9181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 
     9241 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga 
     9301 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 
     9361 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 
     9421 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc 
     9481 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 
     9541 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 
     9601 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 
     9661 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 
     9721 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 
     9781 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 
     9841 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 
     9901 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 
     9961 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 
    10021 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 
    10081 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 
    10141 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 
    10201 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 
    10261 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 
    10321 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 
    10381 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 
    10441 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 
    10501 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 
    10561 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 
    10621 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 
    10681 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 
    10741 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 
    10801 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 
    10861 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 
    10921 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 
    10981 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 
    11041 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 
    11101 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 
    11161 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 
    11221 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 
    11281 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 
    11341 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 
    11401 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 
    11461 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 
    11521 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 
    11581 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 
    11641 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 
    11701 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 
    11761 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 
    11821 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 
    11881 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 
    11941 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 
    12001 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 
    12061 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 
    12121 agggaacaaa agctggagct gc 
//
