LOCUS       pLV-EF1a-hSnail-IRES-Hyg        9964 bp    DNA     circular     23-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     CDS             7885..8745
                     /label=Amp(R)
     promoter        8946..9486
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6490..6724
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7299..7754)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    6927..7140
                     /label=SV40 ori
     misc_feature    5811..6407
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     CDS             4776..5801
                     /label=Hyg
     RBS             4168..4752
                     /label=IRES
     CDS             3369..4163
                     /label=hSnail
     polyA_signal    6796..6926
                     /label=SV40 pA
     intron          2361..3303
                     /label=intron
     misc_feature    1807..1929
                     /label=cPPT
BASE COUNT     2353 a      2592 c      2639 g      2380 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gccgcgctct ttcctcgtca ggaagccctc cgaccccaat cggaagccta 
     3421 actacagcga gctgcaggac tctaatccag agtttacctt ccagcagccc tacgaccagg 
     3481 cccacctgct ggcagccatc ccacctccgg agatcctcaa ccccaccgcc tcgctgccaa 
     3541 tgctcatctg ggactctgtc ctggcgcccc aagcccagcc aattgcctgg gcctcccttc 
     3601 ggctccagga gagtcccagg gtggcagagc tgacctccct gtcagatgag gacagtggga 
     3661 aaggctccca gccccccagc ccaccctcac cggctccttc gtccttctcc tctacttcag 
     3721 tctcttcctt ggaggccgag gcctatgctg ccttcccagg cttgggccaa gtgcccaagc 
     3781 agctggccca gctctctgag gccaaggatc tccaggctcg aaaggccttc aactgcaaat 
     3841 actgcaacaa ggaatacctc agcctgggtg ccctcaagat gcacatccga agccacacgc 
     3901 tgccctgcgt ctgcggaacc tgcgggaagg ccttctctag gccctggctg ctacaaggcc 
     3961 atgtccggac ccacactggc gagaagccct tctcctgtcc ccactgcagc cgtgccttcg 
     4021 ctgaccgctc caacctgcgg gcccacctcc agacccactc agatgtcaag aagtaccagt 
     4081 gccaggcgtg tgctcggacc ttctcccgaa tgtccctgct ccacaagcac caagagtccg 
     4141 gctgctcagg atgtccccgc tgagctagcc cctctccctc ccccccccct aacgttactg 
     4201 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat 
     4261 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc 
     4321 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag 
     4381 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc 
     4441 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac 
     4501 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca 
     4561 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt 
     4621 gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa 
     4681 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat 
     4741 aatatggcca cactagagat ccaccggtcg ccaccatgaa aaagcctgaa ctcaccgcga 
     4801 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct 
     4861 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc 
     4921 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat 
     4981 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct 
     5041 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc 
     5101 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc 
     5161 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg 
     5221 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca 
     5281 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc 
     5341 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg 
     5401 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg 
     5461 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact 
     5521 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca 
     5581 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg 
     5641 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa 
     5701 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg 
     5761 gaaaccgacg ccccagcact cgtccgaggg caaaggaata ggcggccgcg tcgacaatca 
     5821 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 
     5881 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 
     5941 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 
     6001 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 
     6061 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 
     6121 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 
     6181 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 
     6241 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 
     6301 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 
     6361 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg 
     6421 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 
     6481 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt 
     6541 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 
     6601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 
     6661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 
     6721 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 
     6781 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     6841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     6901 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc 
     6961 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     7021 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 
     7081 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc 
     7141 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 
     7201 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 
     7261 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt 
     7321 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 
     7381 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 
     7441 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 
     7501 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 
     7561 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 
     7621 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 
     7681 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 
     7741 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 
     7801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 
     7861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 
     7921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 
     7981 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 
     8041 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 
     8101 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 
     8161 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 
     8221 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 
     8281 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 
     8341 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 
     8401 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 
     8461 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 
     8521 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 
     8581 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 
     8641 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 
     8701 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 
     8761 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 
     8821 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 
     8881 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     8941 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     9001 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     9061 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     9121 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     9181 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     9241 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     9301 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     9361 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     9421 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     9481 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     9541 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 
     9601 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 
     9661 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 
     9721 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 
     9781 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 
     9841 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc 
     9901 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag 
     9961 ctgc 
//
