LOCUS       pLV-EF1a-hSnail-ER-IRES-GFP      10633 bp    DNA     circular     28-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     CDS             8554..9414
                     /label=Amp(R)
     promoter        9615..10155
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             7159..7393
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7968..8423)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    7596..7809
                     /label=SV40 ori
     misc_feature    6480..7076
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     CDS             5750..6469
                     /label=EGFP
     RBS             5143..5726
                     /label=IRES
     CDS             4170..5129
                     /label=ER
     CDS             3369..4160
                     /label=hSnail
     intron          2361..3303
                     /label=intron
     misc_feature    1807..1929
                     /label=cPPT
     polyA_signal    7465..7595
                     /label=SV40 pA
BASE COUNT     2550 a      2823 c      2764 g      2496 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gccgcgctct ttcctcgtca ggaagccctc cgaccccaat cggaagccta 
     3421 actacagcga gctgcaggac tctaatccag agtttacctt ccagcagccc tacgaccagg 
     3481 cccacctgct ggcagccatc ccacctccgg agatcctcaa ccccaccgcc tcgctgccaa 
     3541 tgctcatctg ggactctgtc ctggcgcccc aagcccagcc aattgcctgg gcctcccttc 
     3601 ggctccagga gagtcccagg gtggcagagc tgacctccct gtcagatgag gacagtggga 
     3661 aaggctccca gccccccagc ccaccctcac cggctccttc gtccttctcc tctacttcag 
     3721 tctcttcctt ggaggccgag gcctatgctg ccttcccagg cttgggccaa gtgcccaagc 
     3781 agctggccca gctctctgag gccaaggatc tccaggctcg aaaggccttc aactgcaaat 
     3841 actgcaacaa ggaatacctc agcctgggtg ccctcaagat gcacatccga agccacacgc 
     3901 tgccctgcgt ctgcggaacc tgcgggaagg ccttctctag gccctggctg ctacaaggcc 
     3961 atgtccggac ccacactggc gagaagccct tctcctgtcc ccactgcagc cgtgccttcg 
     4021 ctgaccgctc caacctgcgg gcccacctcc agacccactc agatgtcaag aagtaccagt 
     4081 gccaggcgtg tgctcggacc ttctcccgaa tgtccctgct ccacaagcac caagagtccg 
     4141 gctgctcagg atgtccccgc gcggatccac gaaatgaaat gggtgcttca ggagacatga 
     4201 gggctgccaa cctttggcca agccctcttg tgattaagca cactaagaag aatagccctg 
     4261 ccttgtcctt gacagctgac cagatggtca gtgccttgtt ggatgctgaa ccgcccatga 
     4321 tctattctga atatgatcct tctagaccct tcagtgaagc ctcaatgatg ggcttattga 
     4381 ccaacctagc agatagggag ctggttcata tgatcaactg ggcaaagaga gtgccaggct 
     4441 ttggggactt gaatctccat gatcaggtcc accttctcga gtgtgcctgg ctggagattc 
     4501 tgatgattgg tctcgtctgg cgctccatgg aacacccggg gaagctcctg tttgctccta 
     4561 acttgctcct ggacaggaat caaggtaaat gtgtggaagg catggtggag atctttgaca 
     4621 tgttgcttgc tacgtcaagt cggttccgca tgatgaacct gcagggtgaa gagtttgtgt 
     4681 gcctcaaatc catcattttg cttaattccg gagtgtacac gtttctgtcc agcaccttga 
     4741 agtctctgga agagaaggac cacatccacc gtgtcctgga caagatcaca gacactttga 
     4801 tccacctgat ggccaaagct ggcctgactc tgcagcagca gcatcgccgc ctagctcagc 
     4861 tccttctcat tctttcccat atccggcata tgagtaacaa acgcatggag catctctaca 
     4921 acatgaaatg caagaacgtg gtacccctct atgacctgct cctggagatg ttggatgccc 
     4981 accgccttca tgccccagcc agtcgcatgg gagtgccccc agaggagccc agccagaccc 
     5041 agctggccac caccagctcc acttcagcac attccttaca aacctactac atacccccgg 
     5101 aagcagaggg cttccccaac acgatctgag agctcctact agcccctctc cctccccccc 
     5161 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt 
     5221 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt 
     5281 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa 
     5341 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac 
     5401 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg 
     5461 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt 
     5521 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca 
     5581 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct ttacatgtgt 
     5641 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg 
     5701 aaaaacacga tgataatatg gccacactag agatccaccg gtcgccacca tggtgagcaa 
     5761 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 
     5821 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 
     5881 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 
     5941 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 
     6001 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 
     6061 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 
     6121 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 
     6181 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 
     6241 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 
     6301 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 
     6361 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 
     6421 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcgt 
     6481 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 
     6541 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 
     6601 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 
     6661 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 
     6721 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 
     6781 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 
     6841 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 
     6901 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 
     6961 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 
     7021 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 
     7081 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 
     7141 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt 
     7201 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 
     7261 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 
     7321 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 
     7381 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 
     7441 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 
     7501 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     7561 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 
     7621 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     7681 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 
     7741 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta 
     7801 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 
     7861 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 
     7921 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag 
     7981 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     8041 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     8101 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
     8161 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
     8221 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     8281 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 
     8341 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 
     8401 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct 
     8461 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 
     8521 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 
     8581 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 
     8641 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 
     8701 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 
     8761 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 
     8821 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 
     8881 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 
     8941 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 
     9001 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 
     9061 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 
     9121 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 
     9181 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 
     9241 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 
     9301 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 
     9361 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 
     9421 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 
     9481 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 
     9541 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 
     9601 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 
     9661 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 
     9721 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 
     9781 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 
     9841 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 
     9901 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 
     9961 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 
    10021 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 
    10081 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 
    10141 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 
    10201 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 
    10261 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 
    10321 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 
    10381 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 
    10441 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 
    10501 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 
    10561 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa 
    10621 aagctggagc tgc 
//
