LOCUS pLV-EF1a-hOtx2-IRES-RFP 9727 bp DNA circular 28-JAN-2013 COMMENT COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7648..8508 /label=Amp(R) promoter 8709..9249 /label=pUC ori promoter 7..235 /label=RSV LTR 6253..6487 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(7062..7517) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6690..6903 /label=SV40 ori misc_feature 5574..6170 /label=WPRE promoter 1933..3303 /label=human EF1a promoter RBS 4243..4827 /label=IRES CDS 4854..5564 /label=RFP CDS 3369..4238 /label=hOtx2 polyA_signal 6559..6689 /label=SV40 pA intron 2361..3303 /label=intron misc_feature 1807..1929 /label=cPPT BASE COUNT 2370 a 2481 c 2561 g 2315 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccgccaccat gatgtcttat cttaagcaac cgccttacgc agtcaatggg ctgagtctga 3421 ccacttcggg tatggacttg ctgcacccct ccgtgggcta cccggccacc ccccggaaac 3481 agcgccggga gaggacgacg ttcactcggg cgcagctaga tgtgctggaa gcactgtttg 3541 ccaagacccg gtacccagac atcttcatgc gagaggaggt ggcactgaaa atcaacttgc 3601 ccgagtcgag ggtgcaggta tggtttaaga atcgaagagc taagtgccgc caacaacagc 3661 aacaacagca gaatggaggt caaaacaaag tgagacctgc caaaaagaag acatctccag 3721 ctcgggaagt gagttcagag agtggaacaa gtggccaatt cactcccccc tctagcacct 3781 cagtcccgac cattgccagc agcagtgctc ctgtgtctat ctggagccca gcttccatct 3841 ccccactgtc agatcccttg tccacctcct cttcctgcat gcagaggtcc tatcccatga 3901 cctatactca ggcttcaggt tatagtcaag gatatgctgg ctcaacttcc tactttgggg 3961 gcatggactg tggatcatat ttgaccccta tgcatcacca gcttcccgga ccaggggcca 4021 cactcagtcc catgggtacc aatgcagtca ccagccatct caatcagtcc ccagcttctc 4081 tttccaccca gggatatgga gcttcaagct tgggttttaa ctcaaccact gattgcttgg 4141 attataagga ccaaactgcc tcctggaagc ttaacttcaa tgctgactgc ttggattata 4201 aagatcagac atcctcgtgg aaattccagg ttttgtgatc tagcccctct ccctcccccc 4261 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt 4321 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct 4381 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga 4441 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga 4501 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac 4561 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag 4621 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc 4681 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg 4741 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt 4801 gaaaaacacg atgataatat ggccacacta gagtcgagct accggtcgcc accatggtga 4861 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca 4921 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct 4981 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct 5041 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg 5101 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga 5161 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt 5221 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga 5281 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga 5341 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca 5401 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca 5461 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg 5521 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagcggcc gcgtcgacaa 5581 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 5641 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 5701 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 5761 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 5821 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 5881 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 5941 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 6001 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 6061 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 6121 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc 6181 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 6241 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct 6301 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 6361 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 6421 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 6481 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 6541 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 6601 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 6661 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 6721 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 6781 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 6841 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg 6901 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 6961 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 7021 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 7081 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 7141 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 7201 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 7261 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 7321 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 7381 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 7441 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 7501 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 7561 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 7621 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 7681 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 7741 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 7801 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 7861 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 7921 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 7981 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 8041 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 8101 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 8161 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 8221 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 8281 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 8341 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 8401 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 8461 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 8521 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 8581 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 8641 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 8701 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 8761 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 8821 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 8881 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 8941 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 9001 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 9061 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 9121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 9181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 9241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 9301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 9361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 9421 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 9481 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 9541 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 9601 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 9661 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg 9721 gagctgc //