LOCUS       pLV-EF1a-hOtx2-IRES-Neo        9796 bp    DNA     circular     28-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
            
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7717..8577
                     /label=Amp(R)
     promoter        8778..9318
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6322..6556
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7131..7586)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6628..6758
                     /label=SV40 pA
     misc_feature    6759..6972
                     /label=SV40 ori
     misc_binding    418..435
                     /label=PBS
     misc_feature    5643..6239
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             4839..5633
                     /label=Neo
     RBS             4243..4827
                     /label=IRES
     CDS             3369..4238
                     /label=hOtx2
BASE COUNT     2347 a      2476 c      2585 g      2388 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gatgtcttat cttaagcaac cgccttacgc agtcaatggg ctgagtctga 
     3421 ccacttcggg tatggacttg ctgcacccct ccgtgggcta cccggccacc ccccggaaac 
     3481 agcgccggga gaggacgacg ttcactcggg cgcagctaga tgtgctggaa gcactgtttg 
     3541 ccaagacccg gtacccagac atcttcatgc gagaggaggt ggcactgaaa atcaacttgc 
     3601 ccgagtcgag ggtgcaggta tggtttaaga atcgaagagc taagtgccgc caacaacagc 
     3661 aacaacagca gaatggaggt caaaacaaag tgagacctgc caaaaagaag acatctccag 
     3721 ctcgggaagt gagttcagag agtggaacaa gtggccaatt cactcccccc tctagcacct 
     3781 cagtcccgac cattgccagc agcagtgctc ctgtgtctat ctggagccca gcttccatct 
     3841 ccccactgtc agatcccttg tccacctcct cttcctgcat gcagaggtcc tatcccatga 
     3901 cctatactca ggcttcaggt tatagtcaag gatatgctgg ctcaacttcc tactttgggg 
     3961 gcatggactg tggatcatat ttgaccccta tgcatcacca gcttcccgga ccaggggcca 
     4021 cactcagtcc catgggtacc aatgcagtca ccagccatct caatcagtcc ccagcttctc 
     4081 tttccaccca gggatatgga gcttcaagct tgggttttaa ctcaaccact gattgcttgg 
     4141 attataagga ccaaactgcc tcctggaagc ttaacttcaa tgctgactgc ttggattata 
     4201 aagatcagac atcctcgtgg aaattccagg ttttgtgatc tagcccctct ccctcccccc 
     4261 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt 
     4321 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct 
     4381 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga 
     4441 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga 
     4501 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac 
     4561 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag 
     4621 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc 
     4681 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg 
     4741 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt 
     4801 gaaaaacacg atgataatat ggccacacta gagccaccat gattgaacaa gatggattgc 
     4861 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 
     4921 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 
     4981 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 
     5041 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 
     5101 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 
     5161 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 
     5221 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 
     5281 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 
     5341 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 
     5401 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 
     5461 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 
     5521 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 
     5581 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcggccg 
     5641 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 
     5701 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 
     5761 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 
     5821 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 
     5881 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 
     5941 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 
     6001 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 
     6061 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 
     6121 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 
     6181 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 
     6241 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 
     6301 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg 
     6361 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 
     6421 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 
     6481 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 
     6541 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 
     6601 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 
     6661 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 
     6721 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 
     6781 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     6841 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 
     6901 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta 
     6961 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 
     7021 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 
     7081 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg 
     7141 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 
     7201 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 
     7261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 
     7321 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 
     7381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 
     7441 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 
     7501 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 
     7561 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc 
     7621 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 
     7681 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 
     7741 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 
     7801 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 
     7861 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 
     7921 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 
     7981 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 
     8041 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 
     8101 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 
     8161 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 
     8221 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 
     8281 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 
     8341 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 
     8401 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 
     8461 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 
     8521 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 
     8581 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 
     8641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 
     8701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 
     8761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 
     8821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 
     8881 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 
     8941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 
     9001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 
     9061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 
     9121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 
     9181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 
     9241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 
     9301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 
     9361 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 
     9421 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 
     9481 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 
     9541 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 
     9601 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 
     9661 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 
     9721 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa 
     9781 caaaagctgg agctgc 
//
