LOCUS       pLV-EF1a-hNurr1-IRES-RFP       10654 bp    DNA     circular     28-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
            
FEATURES             Location/Qualifiers
     CDS             8575..9435
                     /label=Amp(R)
     promoter        9636..10176
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             7180..7414
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7989..8444)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    7617..7830
                     /label=SV40 ori
     misc_feature    6501..7097
                     /label=WPRE
     promoter        1933..3303
                     /label=human EF1a promoter
     RBS             5170..5754
                     /label=IRES
     CDS             5781..6491
                     /label=RFP
     CDS             3369..5165
                     /label=hNurr1
     intron          2361..3303
                     /label=intron
     misc_feature    1807..1929
                     /label=cPPT
     polyA_signal    7486..7616
                     /label=SV40 pA
BASE COUNT     2569 a      2792 c      2789 g      2504 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gccttgtgtt caggcgcagt atgggtcctc gcctcaagga gccagccccg 
     3421 cttctcagag ctacagttac cactcttcgg gagaatacag ctccgatttc ttaactccag 
     3481 agtttgtcaa gtttagcatg gacctcacca acactgaaat cactgccacc acttctctcc 
     3541 ccagcttcag tacctttatg gacaactaca gcacaggcta cgacgtcaag ccaccttgct 
     3601 tgtaccaaat gcccctgtcc ggacagcagt cctccattaa ggtagaagac attcagatgc 
     3661 acaactacca gcaacacagc cacctgcccc cccagtctga ggagatgatg ccgcactccg 
     3721 ggtcggttta ctacaagccc tcctcgcccc cgacgcccac caccccgggc ttccaggtgc 
     3781 agcacagccc catgtgggac gacccgggat ctctccacaa cttccaccag aactacgtgg 
     3841 ccactacgca catgatcgag cagaggaaaa cgccagtctc ccgcctctcc ctcttctcct 
     3901 ttaagcaatc gccccctggc accccggtgt ctagttgcca gatgcgcttc gacgggcccc 
     3961 tgcacgtccc catgaacccg gagcccgccg gcagccacca cgtggtggac gggcagacct 
     4021 tcgctgtgcc caaccccatt cgcaagcccg cgtccatggg cttcccgggc ctgcagatcg 
     4081 gccacgcgtc tcagctgctc gacacgcagg tgccctcacc gccgtcgcgg ggctccccct 
     4141 ccaacgaggg gctgtgcgct gtgtgtgggg acaacgcggc ctgccaacac tacggcgtgc 
     4201 gcacctgtga gggctgcaaa ggcttcttta agcgcacagt gcaaaaaaat gcaaaatacg 
     4261 tgtgtttagc aaataaaaac tgcccagtgg acaagcgtcg ccggaatcgc tgtcagtact 
     4321 gccgatttca gaagtgcctg gctgttggga tggtcaaaga agtggttcgc acagacagtt 
     4381 taaaaggccg gagaggtcgt ttgccctcga aaccgaagag cccacaggag ccctctcccc 
     4441 cttcgccccc ggtgagtctg atcagtgccc tcgtcagggc ccatgtcgac tccaacccgg 
     4501 ctatgaccag cctggactat tccaggttcc aggcgaaccc tgactatcaa atgagtggag 
     4561 atgacaccca gcatatccag caattctatg atctcctgac tggctccatg gagatcatcc 
     4621 ggggctgggc agagaagatc cctggcttcg cagacctgcc caaagccgac caagacctgc 
     4681 tttttgaatc agctttctta gaactgtttg tccttcgatt agcatacagg tccaacccag 
     4741 tggagggtaa actcatcttt tgcaatgggg tggtcttgca caggttgcaa tgcgttcgtg 
     4801 gctttgggga atggattgat tccattgttg aattctcctc caacttgcag aatatgaaca 
     4861 tcgacatttc tgccttctcc tgcattgctg ccctggctat ggtcacagag agacacgggc 
     4921 tcaaggaacc caagagagtg gaagaactgc aaaacaagat tgtaaattgt ctcaaagacc 
     4981 acgtgacttt caacaatggg gggttgaacc gccccaatta tttgtccaaa ctgttgggga 
     5041 agctcccaga acttcgtacc ctttgcacac aggggctaca gcgcattttc tacctgaaat 
     5101 tggaagactt ggtgccaccg ccagcaataa ttgacaaact tttcctggac actttacctt 
     5161 tctaatctag cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat 
     5221 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg 
     5281 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc 
     5341 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt 
     5401 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg 
     5461 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac 
     5521 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg 
     5581 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg 
     5641 ggcctcggta cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc 
     5701 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacactagag 
     5761 tcgagctacc ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca 
     5821 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga 
     5881 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga 
     5941 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct 
     6001 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg 
     6061 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc 
     6121 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact 
     6181 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc 
     6241 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg 
     6301 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc 
     6361 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca 
     6421 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc 
     6481 tgtacaagta agcggccgcg tcgacaatca acctctggat tacaaaattt gtgaaagatt 
     6541 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 
     6601 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 
     6661 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 
     6721 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 
     6781 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 
     6841 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 
     6901 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 
     6961 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 
     7021 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 
     7081 ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca 
     7141 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 
     7201 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 
     7261 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
     7321 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
     7381 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 
     7441 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 
     7501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     7561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 
     7621 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 
     7681 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 
     7741 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc 
     7801 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg 
     7861 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 
     7921 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 
     7981 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 
     8041 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 
     8101 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 
     8161 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 
     8221 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 
     8281 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 
     8341 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 
     8401 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 
     8461 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 
     8521 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 
     8581 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 
     8641 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 
     8701 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 
     8761 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 
     8821 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 
     8881 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 
     8941 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 
     9001 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 
     9061 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 
     9121 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 
     9181 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 
     9241 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 
     9301 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 
     9361 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 
     9421 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 
     9481 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 
     9541 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 
     9601 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 
     9661 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 
     9721 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 
     9781 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 
     9841 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 
     9901 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 
     9961 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 
    10021 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 
    10081 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 
    10141 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 
    10201 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 
    10261 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 
    10321 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 
    10381 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 
    10441 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 
    10501 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 
    10561 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 
    10621 aaccctcact aaagggaaca aaagctggag ctgc 
//
