LOCUS       pLV-EF1a-hHNF3b-IRES-RFP       10249 bp    DNA     circular     28-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
            
FEATURES             Location/Qualifiers
     CDS             8170..9030
                     /label=Amp(R)
     promoter        9231..9771
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6775..7009
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7584..8039)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    7212..7425
                     /label=SV40 ori
     misc_feature    6096..6692
                     /label=WPRE
     promoter        1933..3303
                     /label=human EF1a promoter
     RBS             4765..5349
                     /label=IRES
     CDS             5376..6086
                     /label=RFP
     CDS             3369..4760
                     /label=hHNF3b
     intron          2361..3303
                     /label=intron
     polyA_signal    7081..7211
                     /label=SV40 pA
     misc_feature    1807..1929
                     /label=cPPT
BASE COUNT     2417 a      2743 c      2788 g      2301 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gcactcggct tccagtatgc tgggagcggt gaagatggaa gggcacgagc 
     3421 cgtccgactg gagcagctac tatgcagagc ccgagggcta ctcctccgtg agcaacatga 
     3481 acgccggcct ggggatgaac ggcatgaaca cgtacatgag catgtcggcg gccgccatgg 
     3541 gcagcggctc gggcaacatg agcgcgggct ccatgaacat gtcgtcgtac gtgggcgctg 
     3601 gcatgagccc gtccctggcg gggatgtccc ccggcgcggg cgccatggcg ggcatgggcg 
     3661 gctcggccgg ggcggctggc gtggcgggca tggggccgca cttgagtccc agcctgagcc 
     3721 cgctcggggg gcaggcggcc ggggccatgg gcggcctggc cccctacgcc aacatgaact 
     3781 ccatgagccc catgtacggg caggcgggcc tgagccgcgc ccgcgacccc aagacctaca 
     3841 ggcgcagcta cacgcacgca aagccgccct actcgtacat ctcgctcatc accatggcca 
     3901 tccagcagag ccccaacaag atgctgacgc tgagcgagat ctaccagtgg atcatggacc 
     3961 tcttcccctt ctaccggcag aaccagcagc gctggcagaa ctccatccgc cactcgctct 
     4021 ccttcaacga ctgtttcctg aaggtgcccc gctcgcccga caagcccggc aagggctcct 
     4081 tctggaccct gcaccctgac tcgggcaaca tgttcgagaa cggctgctac ctgcgccgcc 
     4141 agaagcgctt caagtgcgag aagcagctgg cgctgaagga ggccgcaggc gccgccggca 
     4201 gcggcaagaa ggcggccgcc ggggcccagg cctcacaggc tcaactcggg gaggccgccg 
     4261 ggccggcctc cgagactccg gcgggcaccg agtcgcctca ctcgagcgcc tccccgtgcc 
     4321 aggagcacaa gcgagggggc ctgggagagc tgaaggggac gccggctgcg gcgctgagcc 
     4381 ccccagagcc ggcgccctct cccgggcagc agcagcaggc cgcggcccac ctgctgggcc 
     4441 cgccccacca cccgggcctg ccgcctgagg cccacctgaa gccggaacac cactacgcct 
     4501 tcaaccaccc gttctccatc aacaacctca tgtcctcgga gcagcagcac caccacagcc 
     4561 accaccacca ccagccccac aaaatggacc tcaaggccta cgaacaggtg atgcactacc 
     4621 ccggctacgg ttcccccatg cctggcagct tggccatggg cccggtcacg aacaaaacgg 
     4681 gcctggacgc ctcgcccctg gccgcagata cctcctacta ccagggggtg tactcccggc 
     4741 ccattatgaa ctcctcttaa tctagcccct ctccctcccc cccccctaac gttactggcc 
     4801 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc 
     4861 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta 
     4921 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag 
     4981 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga 
     5041 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg 
     5101 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat 
     5161 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta 
     5221 tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa 
     5281 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat 
     5341 atggccacac tagagtcgag ctaccggtcg ccaccatggt gagcaagggc gaggaggata 
     5401 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg 
     5461 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg 
     5521 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc 
     5581 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga 
     5641 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg 
     5701 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc 
     5761 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg 
     5821 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga 
     5881 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca 
     5941 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc 
     6001 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg 
     6061 gcggcatgga cgagctgtac aagtaagcgg ccgcgtcgac aatcaacctc tggattacaa 
     6121 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 
     6181 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 
     6241 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 
     6301 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 
     6361 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 
     6421 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 
     6481 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 
     6541 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 
     6601 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 
     6661 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca 
     6721 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 
     6781 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 
     6841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 
     6901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 
     6961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 
     7021 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 
     7081 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     7141 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     7201 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 
     7261 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 
     7321 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 
     7381 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 
     7441 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 
     7501 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 
     7561 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     7621 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     7681 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     7741 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     7801 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     7861 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     7921 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 
     7981 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 
     8041 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 
     8101 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 
     8161 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 
     8221 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 
     8281 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 
     8341 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 
     8401 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 
     8461 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 
     8521 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 
     8581 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 
     8641 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 
     8701 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 
     8761 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 
     8821 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 
     8881 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 
     8941 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
     9001 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
     9061 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     9121 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     9181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     9241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     9301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 
     9361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     9421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     9481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     9541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     9601 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     9661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     9721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     9781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     9841 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 
     9901 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 
     9961 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 
    10021 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 
    10081 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 
    10141 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 
    10201 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc 
//
