LOCUS       pLV-EF1a-hHIF2a-IRES-Neo       11584 bp    DNA     circular     28-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     CDS             9505..10365
                     /label=Amp(R)
     promoter        10566..11106
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     intron          526..1690
                     /label=Intron
     rep_origin      complement(8919..9374)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    8547..8760
                     /label=SV40 ori
     misc_feature    7431..8027
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     CDS             6627..7421
                     /label=Neo
     RBS             6031..6615
                     /label=IRES
     CDS             3369..5981
                     /label=hHIF2a
     LTR             8110..8344
                     /label=3' UTR
     polyA_signal    8416..8546
                     /label=SV40 pA
     misc_feature    1807..1929
                     /label=cPPT
     intron          2361..3303
                     /label=intron
BASE COUNT     2779 a      3035 c      3090 g      2680 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gacagctgac aaggagaaga aaaggagtag ctcggagagg aggaaggaga 
     3421 agtcccggga tgctgcgcgg tgccggcgga gcaaggagac ggaggtgttc tatgagctgg 
     3481 cccatgagct gcctctgccc cacagtgtga gctcccatct ggacaaggcc tccatcatgc 
     3541 gactggcaat cagcttcctg cgaacacaca agctcctctc ctcagtttgc tctgaaaacg 
     3601 agtccgaagc cgaagctgac cagcagatgg acaacttgta cctgaaagcc ttggagggtt 
     3661 tcattgccgt ggtgacccaa gatggcgaca tgatctttct gtcagaaaac atcagcaagt 
     3721 tcatgggact tacacaggtg gagctaacag gacatagtat ctttgacttc actcatccct 
     3781 gcgaccatga ggagattcgt gagaacctga gtctcaaaaa tggctctggt tttgggaaaa 
     3841 aaagcaaaga catgtccaca gagcgggact tcttcatgag gatgaagtgc acggtcacca 
     3901 acagaggccg tactgtcaac ctcaagtcag ccacctggaa ggtcttgcac tgcacgggcc 
     3961 aggtgaaagt ctacaacaac tgccctcctc acaatagtct gtgtggctac aaggagcccc 
     4021 tgctgtcctg cctcatcatc atgtgtgaac caatccagca cccatcccac atggacatcc 
     4081 ccctggatag caagaccttc ctgagccgcc acagcatgga catgaagttc acctactgtg 
     4141 atgacagaat cacagaactg attggttacc accctgagga gctgcttggc cgctcagcct 
     4201 atgaattcta ccatgcgcta gactccgaga acatgaccaa gagtcaccag aacttgtgca 
     4261 ccaagggtca ggtagtaagt ggccagtacc ggatgctcgc aaagcatggg ggctacgtgt 
     4321 ggctggagac ccaggggacg gtcatctaca accctcgcaa cctgcagccc cagtgcatca 
     4381 tgtgtgtcaa ctacgtcctg agtgagattg agaagaatga cgtggtgttc tccatggacc 
     4441 agactgaatc cctgttcaag ccccacctga tggccatgaa cagcatcttt gatagcagtg 
     4501 gcaagggggc tgtgtctgag aagagtaact tcctattcac caagctaaag gaggagcccg 
     4561 aggagctggc ccagctggct cccaccccag gagacgccat catctctctg gatttcggga 
     4621 atcagaactt cgaggagtcc tcagcctatg gcaaggccat cctgcccccg agccagccat 
     4681 gggccacgga gttgaggagc cacagcaccc agagcgaggc tgggagcctg cctgccttca 
     4741 ccgtgcccca ggcagctgcc ccgggcagca ccacccccag tgccaccagc agcagcagca 
     4801 gctgctccac gcccaatagc cctgaagact attacacatc tttggataac gacctgaaga 
     4861 ttgaagtgat tgagaagctc ttcgccatgg acacagaggc caaggaccaa tgcagtaccc 
     4921 agacggattt caatgagctg gacttggaga cactggcacc ctatatcccc atggacgggg 
     4981 aagacttcca gctaagcccc atctgccccg aggagcggct cttggcggag aacccacagt 
     5041 ccacccccca gcactgcttc agtgccatga caaacatctt ccagccactg gcccctgtag 
     5101 ccccgcacag tcccttcctc ctggacaagt ttcagcagca gctggagagc aagaagacag 
     5161 agcccgagca ccggcccatg tcctccatct tctttgatgc cggaagcaaa gcatccctgc 
     5221 caccgtgctg tggccaggcc agcacccctc tctcttccat ggggggcaga tccaataccc 
     5281 agtggccccc agatccacca ttacattttg ggcccacaaa gtgggccgtc ggggatcagc 
     5341 gcacagagtt cttgggagca gcgccgttgg ggccccctgt ctctccaccc catgtctcca 
     5401 ccttcaagac aaggtctgca aagggttttg gggctcgagg cccagacgtg ctgagtccgg 
     5461 ccatggtagc cctctccaac aagctgaagc tgaagcgaca gctggagtat gaagagcaag 
     5521 ccttccagga cctgagcggg ggggacccac ctggtggcag cacctcacat ttgatgtgga 
     5581 aacggatgaa gaacctcagg ggtgggagct gccctttgat gccggacaag ccactgagcg 
     5641 caaatgtacc caatgataag ttcacccaaa accccatgag gggcctgggc catcccctga 
     5701 gacatctgcc gctgccacag cctccatctg ccatcagtcc cggggagaac agcaagagca 
     5761 ggttcccccc acagtgctac gccacccagt accaggacta cagcctgtcg tcagcccaca 
     5821 aggtgtcagg catggcaagc cggctgctcg ggccctcatt tgagtcctac ctgctgcccg 
     5881 aactgaccag atatgactgt gaggtgaacg tgcccgtgct gggaagctcc acgctcctgc 
     5941 aaggagggga cctcctcaga gccctggacc aggccacctg acaattctgc agatatccat 
     6001 cacactggcg gccgctcgag catgcatcta gcccctctcc ctcccccccc cctaacgtta 
     6061 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca 
     6121 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca 
     6181 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg 
     6241 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc 
     6301 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata 
     6361 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag 
     6421 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc 
     6481 attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt 
     6541 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat 
     6601 gataatatgg ccacactaga gccaccatga ttgaacaaga tggattgcac gcaggttctc 
     6661 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 
     6721 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 
     6781 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 
     6841 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 
     6901 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 
     6961 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 
     7021 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 
     7081 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 
     7141 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 
     7201 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 
     7261 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 
     7321 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 
     7381 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcggccgcg tcgacaatca 
     7441 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 
     7501 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 
     7561 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 
     7621 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 
     7681 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 
     7741 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 
     7801 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 
     7861 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 
     7921 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 
     7981 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg 
     8041 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 
     8101 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt 
     8161 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 
     8221 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 
     8281 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 
     8341 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 
     8401 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     8461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     8521 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc 
     8581 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     8641 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 
     8701 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc 
     8761 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 
     8821 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 
     8881 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt 
     8941 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 
     9001 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 
     9061 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 
     9121 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 
     9181 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 
     9241 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 
     9301 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 
     9361 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 
     9421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 
     9481 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 
     9541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 
     9601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 
     9661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 
     9721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 
     9781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 
     9841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 
     9901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 
     9961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 
    10021 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 
    10081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 
    10141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 
    10201 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 
    10261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 
    10321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 
    10381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 
    10441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 
    10501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
    10561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
    10621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
    10681 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
    10741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
    10801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
    10861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
    10921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
    10981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
    11041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
    11101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
    11161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 
    11221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 
    11281 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 
    11341 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 
    11401 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 
    11461 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc 
    11521 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag 
    11581 ctgc 
//
